Mohamed Sara E Ibrahim, Ahmed Romaz M, Jawasreh Khaleel I Z, Salih M A M, Abdelhalim Dalia Mursi, Abdelgadir A W, Obeidat Md T, Musa L M A, Ahmed Mohammed-Khair A
Animal Production Research Centre, Animal Resources Research Corporation, Ministry of Animal Resources, Khartoum, Sudan.
Institute for Studies and Promotion of Animal Exports, University of Khartoum, Khartoum, Sudan.
Vet World. 2019 Apr;13(4):614-621. doi: 10.14202/vetworld.2020.614-621. Epub 2020 Apr 5.
The Watish sheep is a strain of desert sheep of smaller size compared to other desert sheep ecotypes, and there is anecdotal evidence that it is endowed with high litter size. The present study was designed for screening for polymorphisms in the known fecundity genes (bone morphogenetic protein receptor type 1B A<G in exon 6, bone morphogenetic protein 15 () ( , , , and ) in exon2, growth differentiation factor 9 () - G1 in exon1 and G8 in exon2 and PRLG<A in intron2) and their association with litter size in Watish.
The study involved 156 Watish ewes of 2-6 years of age, along with data on litter size in the first, second, and third parity from Sinnar state and contiguous Blue Nile State. Genomic DNA was isolated and genotyped using polymerase chain reaction-restriction fragment length polymorphism. Allele and genotype frequencies were calculated by direct counting. Chi-square test for goodness of fit was performed for agreement with Hardy-Weinberg expectations and association testing.
The results demonstrated that all individuals were non-carriers for the target mutations of , ( , and ), and -G8. With regard to the -G1 gene, the genotypic frequencies were 0.07% (G+) and 0.93% (++), in gene they were 0.993% (++) and 0.006% (B+), in gene 0.516(++), 0.347(B+), and 0.137(BB). The Chi-square test showed a non-significant association between ewe's type of birth and the detected mutations genotypes.
These results preliminarily indicated that -G1, ( ), and genes might have had some contribution for improving litter size in Watish Sudanese sheep. However, further studies using larger samples are needed to detect the effects of those mutations on Watish sheep litter size.
瓦提什绵羊是一种沙漠绵羊品种，体型比其他沙漠绵羊生态型小，有传闻称其产仔数高。本研究旨在筛选已知繁殖力基因（骨形态发生蛋白受体1B基因第6外显子A<G、骨形态发生蛋白15基因（ 、 、 、 ）第2外显子、生长分化因子9基因第1外显子-G1和第2外显子G8以及催乳素基因内含子2中P<G）的多态性及其与瓦提什绵羊产仔数的关联。
本研究涉及156只2至6岁的瓦提什母羊，以及来自森纳尔州和相邻的青尼罗州的头胎、二胎和三胎产仔数数据。提取基因组DNA，采用聚合酶链反应-限制性片段长度多态性方法进行基因分型。通过直接计数计算等位基因和基因型频率。进行卡方拟合优度检验以符合哈迪-温伯格预期并进行关联测试。
结果表明，所有个体均不是 、 （ 、 和 ）以及-G8目标突变的携带者。关于-G1基因，基因型频率分别为0.07%（G+）和0.93%（++）； 基因中，分别为0.993%（++）和0.006%（B+）； 基因中，分别为0.516（++）、0.347（B+）和0.137（BB）。卡方检验表明，母羊的出生类型与检测到的突变基因型之间无显著关联。
这些结果初步表明，-G1、 （ ）和 基因可能对提高苏丹瓦提什绵羊的产仔数有一定作用。然而，需要使用更大样本进行进一步研究，以检测这些突变对瓦提什绵羊产仔数的影响。
