Araujo-Pereira Thais de, Pita-Pereira Daniela de, Boité Mariana Côrtes, Melo Myllena, Costa-Rego Taiana Amancio da, Fuzari Andressa Alencastre, Brazil Reginaldo Peçanha, Britto Constança
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Pesquisa em Leishmaniose, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2017 Jan 1;112(1):75-78. doi: 10.1590/0074-02760160283.
Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subgenus in 12 out of 173 sandflies captured in the municipality of Rio Branco, Acre state, revealing a positivity of 6.94%. By sequencing the Leishmania 234 bp-hsp70 amplified products from positive samples, infection by L. (V.) braziliensis was confirmed in five sandflies: one Evandromyia saulensis, three Trichophoromyia auraensis and one Pressatia sp. The finding of L. (Viannia) DNA in two Ev. saulensis corresponds to the first record of possible infection associated with this sandfly. Moreover, our study reveals for the first time in Brazil, Th. auraensis and Pressatia sp. infected by L. (Viannia) parasites.
在皮肤利什曼病发病率不断上升的巴西亚马逊地区，关于白蛉动物区系以评估自然感染指标的研究仍然有限。在此，通过使用针对利什曼原虫kDNA的多重聚合酶链反应和杂交技术，我们在阿克里州里奥布兰科市捕获的173只白蛉中的12只中鉴定出了维氏亚属，阳性率为6.94%。通过对阳性样本中扩增出的利什曼原虫234 bp - hsp70产物进行测序，在五只白蛉中确认感染了巴西利什曼原虫：一只索尔伊蚊、三只奥拉伊蚊和一只普雷萨蒂亚属。在两只索尔伊蚊中发现维氏亚属DNA，这是与该白蛉相关的可能感染的首次记录。此外，我们的研究首次在巴西揭示了奥拉伊蚊和普雷萨蒂亚属感染了维氏亚属寄生虫。
