Göncü Tuğba, Oğuz Elif, Sezen Hatice, Koçarslan Sezen, Oğuz Halit, Akal Ali, Adıbelli Fatih Mehmet, Çakmak Sevim, Aksoy Nurten
Department of Ophthalmology, Harran University, School of Medicine, Sanliurfa, Turkey.
Department of Medical Pharmacology, School of Medicine, Harran University, Sanliurfa, Turkey.
Arq Bras Oftalmol. 2016 Nov-Dec;79(6):357-362. doi: 10.5935/0004-2749.20160102.
: We evaluated the efficacy of lycopene, a dietary carotenoid and potent antioxidant, against ocular inflammation and oxidative stress in an experimental uveitis model.
: Endotoxin-induced uveitis (EIU) was induced in Sprague-Dawley rats by a single subcutaneous injection of 200 μg lipopolysaccharide (LPS). Induction of EIU was preceded by daily intraperitoneal injection of 10 mg/kg lycopene for three consecutive days (Lycopene + LPS group) or equivolume vehicle (Vehicle + LPS group). A positive control group received 1 mg/kg dexamethasone pretreatment (DEX + LPS), and a negative control group received daily vehicle injection but no LPS (Vehicle Control). Twenty-four hours after LPS or final vehicle administration, eyes were enucleated, and aqueous humor was collected for measurement of the number of infiltrating cells, total protein concentration, and levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and oxidative stress markers. Inflammatory response severity was compared among groups clinically and histopathologically.
: Infiltrating cell number, total protein concentration, and NO, TNF-α, and IL-6 levels were significantly elevated in the aqueous humor of Vehicle + LPS group rats compared to Vehicle Controls. Compared to the Vehicle + LPS group, lycopene pretreatment significantly reduced aqueous humor concentrations of oxidative stress markers, NO (0.29 ± 0.1 μM vs. 0.19 ± 0.1 μM, p=0.003), TNF-α (71.0 ± 22.3 ng/ml vs. 50.1 ± 2.1 ng/ml, p=0.043), and IL-6 (121.6 ± 3.0 pg/ml vs. 111.1 ± 5.6 pg/ml, p=0.008). Inflammatory score was also reduced (2.0 ± 0.0 vs. 0.4 ± 0.5, p=0.001). Lycopene reduced the infiltrating cell count and protein concentration, but differences did not reach significance. Most lycopene effects were equivalent to dexamethasone.
: Lycopene may aid in the clinical management of uveitis by suppressing inflammation and oxidative stress.
我们评估了番茄红素（一种膳食类胡萝卜素和强效抗氧化剂）在实验性葡萄膜炎模型中对抗眼部炎症和氧化应激的功效。
通过单次皮下注射200μg脂多糖（LPS）在Sprague-Dawley大鼠中诱导内毒素性葡萄膜炎（EIU）。在诱导EIU之前，连续三天每天腹腔注射10mg/kg番茄红素（番茄红素+LPS组）或等体积的载体（载体+LPS组）。阳性对照组接受1mg/kg地塞米松预处理（DEX+LPS），阴性对照组每天注射载体但不注射LPS（载体对照）。在给予LPS或最后一次载体后24小时，摘除眼球，收集房水以测量浸润细胞数量、总蛋白浓度以及一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和氧化应激标志物的水平。在临床和组织病理学上比较各组之间的炎症反应严重程度。
与载体对照组相比，载体+LPS组大鼠房水中的浸润细胞数量、总蛋白浓度以及NO、TNF-α和IL-6水平显著升高。与载体+LPS组相比，番茄红素预处理显著降低了房水中氧化应激标志物、NO（0.29±0.1μM对0.19±0.1μM，p=0.003）、TNF-α（71.0±22.3ng/ml对50.1±2.1ng/ml，p=0.043）和IL-6（121.6±3.0pg/ml对111.1±5.6pg/ml，p=0.008）的浓度。炎症评分也降低了（2.0±0.0对0.4±0.5，p=0.001）。番茄红素降低了浸润细胞计数和蛋白浓度，但差异未达到显著水平。大多数番茄红素的作用与地塞米松相当。
番茄红素可能通过抑制炎症和氧化应激有助于葡萄膜炎的临床管理。
