Ghasemnezhad Targhi Reza, Saba Valiallah
Department of Radiology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Radiology, Faculty of Para medicine, AJA University of Medical Sciences, Tehran, Iran.
Avicenna J Phytomed. 2020 Jul-Aug;10(4):398-406.
Ionizing radiation induces deleterious effects in the biological systems by producing free radicals. Grape Seed Extract (GSE) as a free radical scavenger could protect the body against the damages
In this study, 12 healthy male volunteers were divided into Groups 1, 2, 3 and 4 and received 100, 300, 600 and 1000 mg GSE, respectively. Peripheral blood samples were collected from each volunteer 15 min before, and 1, 2, and 5 hr after GSE oral administration. Blood samples were then irradiated with 150 cGy of 100 kvp X-ray (Irradiated control group, was treated with only 1.5 Gy of X-rays). Cytogenic damages were detected by micronucleus assay.
Results showed that irradiation significantly increased the incidence of micronuclei (p<0. 001). In group 1, the mean reduction of micronucleus rate was 26.53%, 34.92%, and 31.38%, 1, 2, and 5 hr after GSE ingestion (p<0.001), respectively; this variable in group 2 was 17.38, 38.33, and 31.38 (p<0. 001), in group 3, was 35.65%, 46%, and 37.15% (p<0.001), respectively and in group 4, was 41.35%, 51.73%, and 50.55% (p<0.0001), respectively. The samples collected 1, 2, and 5 hr after ingestion of GSE exhibited a significant decrease in the incidence of micronuclei compared with the radiation control group. The maximum protection and reduction in frequency of micronuclei (51.73%) was observed 2 hr after ingestion of 1000 mg GSE.
Consumption of GSE before undergoing radiation protects human lymphocytes against X-rays by reducing radiation-induced genotoxicity.
电离辐射通过产生自由基在生物系统中诱导有害效应。葡萄籽提取物（GSE）作为一种自由基清除剂可保护身体免受损伤。
在本研究中，12名健康男性志愿者被分为1、2、3和4组，分别接受100、300、600和1000毫克GSE。在口服GSE前15分钟以及口服后1、2和5小时从每名志愿者采集外周血样本。然后将血样用100千伏峰值的150厘戈瑞X射线进行照射（照射对照组仅用1.5戈瑞X射线进行处理）。通过微核试验检测细胞遗传学损伤。
结果显示，照射显著增加了微核发生率（p<0.001）。在第1组中，摄入GSE后1、2和5小时微核率的平均降低分别为26.53%、34.92%和31.38%（p<0.001）；第2组中该变量分别为17.38%、38.33%和31.38%（p<0.001），第3组分别为35.65%、46%和37.15%（p<0.001），第4组分别为41.35%、51.73%和50.55%（p<0.0001）。与辐射对照组相比，摄入GSE后1、2和5小时采集的样本微核发生率显著降低。摄入1000毫克GSE后2小时观察到最大程度的保护以及微核频率降低（51.73%）。
在接受辐射前食用GSE可通过降低辐射诱导的遗传毒性来保护人类淋巴细胞免受X射线损伤。
