Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu 300, Taiwan.
Department of Dermatology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
Molecules. 2018 Aug 9;23(8):1987. doi: 10.3390/molecules23081987.
Acne vulgaris (acne) is a common inflammatory skin disorder, and plays a major role in the development and progression of acne inflammation. Herbs possessing antimicrobial and anti-inflammatory activity have been applied as a medical option for centuries. In this study, we examined the suppressive effect of ethanolic oregano () extract on live -induced in vivo and in vitro inflammation. Following ethanol extraction of oregano leaves, four compounds with strong antioxidant activity, including rosmarinic acid, quercetin, apigenin, and carvacrol, were identified by high-performance liquid chromatography. Using the mouse ear edema model, we demonstrated that ethanol oregano extracts (EOE) significantly suppressed -induced skin inflammation, as measured by ear thickness (32%) and biopsy weight (37%). In a separate study, using the co-culture of and human THP-1 monocytes, EOE reduced the production of interleukin (IL)-8, IL-1β and tumor necrosis factor (TNF)-α up to 40%, 37%, and 18%, respectively, as well as the expression of these three pro-inflammatory mediators at the transcriptional level. Furthermore, EOE inhibited the translocation of nuclear factor-kappa B (NF-κB) into the nucleus possibly by inactivating toll-like receptor-2 (TLR2). The suppressive effect of EOE on live -induced inflammatory responses could be due, in part, to the anti-inflammatory and antioxidant properties, but not the anti-microbial effect of EOE.
寻常痤疮（痤疮）是一种常见的炎症性皮肤病，在痤疮炎症的发展和进展中起主要作用。具有抗菌和抗炎活性的草药已被应用于医疗数百年。在这项研究中，我们研究了乙醇牛至（）提取物对活诱导的体内和体外炎症的抑制作用。在对牛至叶进行乙醇提取后，通过高效液相色谱法鉴定了四种具有强抗氧化活性的化合物，包括迷迭香酸、槲皮素、芹菜素和香芹酚。使用小鼠耳肿胀模型，我们证明乙醇牛至提取物（EOE）显著抑制了由诱导的皮肤炎症，表现为耳厚度（32%）和活检重量（37%）降低。在另一项研究中，通过和人 THP-1 单核细胞的共培养，EOE 将白细胞介素（IL）-8、IL-1β和肿瘤坏死因子（TNF）-α的产生分别降低了 40%、37%和 18%，并且这些三种促炎介质的表达在转录水平上也降低了。此外，EOE 通过使 Toll 样受体-2（TLR2）失活，抑制核因子-κB（NF-κB）向核内的易位。EOE 对活诱导的炎症反应的抑制作用可能部分归因于其抗炎和抗氧化特性，而不是 EOE 的抗菌作用。
