Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Sci Rep. 2017 Jan 12;7:40494. doi: 10.1038/srep40494.
Some herbivore-induced-plant volatiles (HIPVs) compounds are vital for the functioning of an ecosystem, by triggering multi-trophic interactions for natural enemies, plants and herbivores. However, the effect of these chemicals, which play a crucial role in regulating the multi-trophic interactions between plant-herbivore-entomopathogenic fungi, is still unknown. To fill this scientific gap, we therefore investigated how these chemicals influence the entomopathogenic fungi growth and efficacy. In this study, Lipaphis erysimi induced Arabidopsis thaliana HIPVs were collected using headspace system and detected with GC-MS, and then analyzed the effects of these HIPVs chemicals on Lecanicillium lecanii strain V3450. We found that the HIPVs menthol and methyl salicylate at 1 and 10 nmol·ml improved many performance aspects of the fungus, such as germination, sporulation, appressorial formation as well as its pathogenicity and virulence. These findings are not only important for understanding the multi-trophic interactions in an ecosystem, but also would contribute for developing new and easier procedures for conidial mass production as well as improve the pathogenicity and virulence of entomopathogenic fungi in biological pest management strategies.
一些草食性诱导植物挥发物（HIPVs）化合物对于生态系统的功能至关重要，它们可以引发天敌、植物和草食性动物之间的多营养级相互作用。然而，这些在调节植物-草食性动物-昆虫病原真菌之间的多营养级相互作用中起着关键作用的化学物质的作用仍然未知。为了填补这一科学空白，我们因此研究了这些化学物质如何影响昆虫病原真菌的生长和功效。在这项研究中，我们使用顶空系统收集了甘蓝蚜诱导的拟南芥 HIPVs，并使用 GC-MS 进行检测，然后分析了这些 HIPVs 化学物质对 Lecanicillium lecanii 菌株 V3450 的影响。我们发现，1 和 10nmol·ml 的 HIPVs 薄荷醇和甲基水杨酸可改善真菌的许多性能方面，如萌发、产孢、附着胞形成以及其致病性和毒力。这些发现不仅对理解生态系统中的多营养级相互作用很重要，而且有助于开发新的、更容易的孢子大规模生产程序，并提高昆虫病原真菌在生物防治策略中的致病性和毒力。
