Center for Chromatography and Mass Spectrometry (CROM-MASS), Universidad Industrial de Santander, Bucaramanga 680002, Colombia.
Colombia Research Center for Biomolecules (CIBIMOL), Universidad Industrial de Santander, Bucaramanga 680002, Colombia.
Molecules. 2021 Nov 17;26(22):6941. doi: 10.3390/molecules26226941.
Plants are known to increase the emission of volatile organic compounds upon the damage of phytophagous insects. However, very little is known about the composition and temporal dynamics of volatiles released by wild plants of the genus (Fabaceae) attacked with the specialist lepidopteran caterpillar (Linnaeus) (Erebidae). In this work, the herbivore-induced plant volatiles (HIPV) emitted by Kunth plants were isolated with solid phase micro-extraction and the conventional purge and trap technique, and their identification was carried out by GC/MS. The poly-dimethylsiloxane/divinylbenzene fiber showed higher affinity for the extraction of apolar compounds (e.g., -β-caryophyllene) compared to the Porapak™-Q adsorbent from the purge & trap method that extracted more polar compounds (e.g., -nerolidol and indole). The compounds emitted by were mainly green leaf volatile substances, terpenoids, aromatics, and aldoximes (isobutyraldoxime and 2-methylbutyraldoxime), whose maximum emission was six hours after the attack. The attack by caterpillars significantly increased the volatile compounds emission in the leaves compared to those subjected to mechanical damage. This result indicated that the caterpillar is responsible for generating a specific response in plants. It was demonstrated that HIPVs repelled conspecific moths from attacked plants and favored oviposition in those without damage. The results showed the importance of volatiles in plant-insect interactions, as well as the choice of appropriate extraction and analytical methods for their study.
植物在受到植食性昆虫的伤害时，会增加挥发性有机化合物的排放。然而，对于被专食性鳞翅目毛毛虫（Linnaeus）（夜蛾科）攻击的野生植物（豆科）释放的挥发性化合物的组成和时间动态，人们知之甚少。在这项工作中，使用固相微萃取和常规吹扫捕集技术分离了昆特植物的植物诱导挥发性物质（HIPV），并通过 GC/MS 对其进行了鉴定。与从吹扫捕集法中提取更多极性化合物（例如 -nerolidol 和吲哚）的 Porapak™-Q 吸附剂相比，聚二甲基硅氧烷/二乙烯基苯纤维对非极性化合物（例如 -β-石竹烯）的提取具有更高的亲和力。由 释放的化合物主要是绿叶挥发性物质、萜烯、芳烃和醛肟（异丁醛肟和 2-甲基丁醛肟），其最大排放量在受到攻击后六个小时。与机械损伤相比，毛毛虫的攻击显著增加了 叶片中挥发性化合物的排放。这一结果表明，毛毛虫负责在 植物中产生特定的反应。结果表明，HIPVs 排斥来自受攻击植物的同种飞蛾，并有利于在未受损的植物中产卵。结果表明了挥发性物质在植物-昆虫相互作用中的重要性，以及选择适当的提取和分析方法来研究它们的重要性。
