Alaraby Mohamed, Hernández Alba, Marcos Ricard
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.
Zoology Department, Faculty of Sciences, Sohag University, Sohag, Egypt.
Environ Mol Mutagen. 2017 Jan;58(1):46-55. doi: 10.1002/em.22068. Epub 2017 Jan 12.
The biological reactivity of metal and metal oxide nanomaterials is attributed to their redox properties, which would explain their pro- or anti-cancer properties depending on exposure circumstances. In this sense, copper oxide nanoparticles (CuONP) have been proposed as a potential anti-tumoral agent. The aim of this study was to assess if CuONP can exert antigenotoxic effects using Drosophila melanogaster as an in vivo model. Genotoxicity was induced by two well-known genotoxic compounds, namely potassium dichromate (PD) and ethyl methanesulfonate (EMS). The wing-spot assay and the comet assay were used as biomarkers of genotoxic effects. In addition, changes in the expression of Ogg1 and Sod genes were determined. The effects of CuONP cotreatment were compared with those induced by copper sulfate (CS), an agent releasing copper ions. Using the wing-spot assay, CuONP and CS were not able to reduce the genotoxic effects of EMS exposure, but had the ability to decrease the effects induced by PD, reducing the frequency of mutant twin-spots that arise from mitotic recombination. In addition, CuONP and CS were able to reduce the DNA damage induced by PD as determined by the comet assay. In general, similar qualitative antigenotoxic effects were obtained with both copper compounds. The antigenotoxic effects of environmentally relevant and non-toxic doses of CuONP and CS may be explained by their ability to partially restore the expression levels of the repair gene Ogg1 and the antioxidant gene Cu,ZnSod, both of which are inhibited by PD treatment. Environ. Mol. Mutagen. 58:46-55, 2017. © 2016 Wiley Periodicals, Inc.
金属及金属氧化物纳米材料的生物反应活性归因于其氧化还原特性，这可以解释它们在不同暴露环境下的促癌或抗癌特性。从这个意义上讲，氧化铜纳米颗粒（CuONP）已被提议作为一种潜在的抗肿瘤剂。本研究的目的是使用黑腹果蝇作为体内模型，评估CuONP是否能发挥抗基因毒性作用。通过两种著名的基因毒性化合物，即重铬酸钾（PD）和甲基磺酸乙酯（EMS）诱导基因毒性。翅斑试验和彗星试验被用作基因毒性效应的生物标志物。此外，还测定了Ogg1和Sod基因表达的变化。将CuONP联合处理的效果与硫酸铜（CS，一种释放铜离子的试剂）诱导的效果进行比较。使用翅斑试验，CuONP和CS不能降低EMS暴露的基因毒性效应，但有能力降低PD诱导的效应，减少由有丝分裂重组产生的突变双斑频率。此外，通过彗星试验测定，CuONP和CS能够降低PD诱导的DNA损伤。一般来说，两种铜化合物都获得了类似的定性抗基因毒性效果。环境相关且无毒剂量的CuONP和CS的抗基因毒性作用可能是由于它们能够部分恢复修复基因Ogg1和抗氧化基因Cu,ZnSod的表达水平，这两个基因都受到PD处理的抑制。《环境与分子突变》58:46 - 55, 2017。© 2016威利期刊公司
