Keshavarzi Majid, Khodaei Forouzan, Siavashpour Asma, Saeedi Arastoo, Mohammadi-Bardbori Afshin
Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Pharm Res. 2019 Fall;18(4):2042-2054. doi: 10.22037/ijpr.2019.13971.12030.
The concerns about the possible risk of manufactured nanoparticles (NPs) have been raised recently. Nano- and micro-sized copper oxide (CO and CONP) are widely used in many industries. In this regard, studies have demonstrated that CONP is a toxic compound in different cell lines. Despite their unique properties, NPs possess unexpected toxicity profiling relative to the bulk materials. This study was designed to examine and compare the toxic effects of CO and CONPs and in isolated rat mitochondria. Male Wistar albino rats received 50 to 1000 mg/kg CO or CONP by gavage and several toxicological endpoints including biochemical indices and oxidative stress markers. Then, the pathological parameters in the multiple organs such as liver, brain, spleen, kidney, and intestine were assessed. Mitochondria were isolated from the rat liver and several mitochondrial indices were measured. The results of this study demonstrated that CO and CONP exhibited biphasic dose-response effects. CONPs showed higher toxicity compared with the bulk material. There were no significant changes in the results of CONP and CO in isolated rat liver mitochondria. The present studies provided more information regarding the hormetic effects of CO and CONPs and in isolated rat mitochondria.
最近人们对人造纳米颗粒（NPs）可能存在的风险表示担忧。纳米和微米级的氧化铜（CO和CONP）在许多行业中广泛使用。在这方面，研究表明CONP在不同细胞系中是一种有毒化合物。尽管纳米颗粒具有独特的性质，但相对于块状材料，它们具有意想不到的毒性特征。本研究旨在检查和比较CO和CONP对分离的大鼠线粒体的毒性作用。雄性Wistar白化大鼠通过灌胃接受50至1000 mg/kg的CO或CONP，并检测包括生化指标和氧化应激标志物在内的几个毒理学终点。然后，评估肝脏、大脑、脾脏、肾脏和肠道等多个器官的病理参数。从大鼠肝脏中分离出线粒体，并测量几个线粒体指标。本研究结果表明，CO和CONP表现出双相剂量反应效应。与块状材料相比，CONP显示出更高的毒性。在分离的大鼠肝脏线粒体中，CONP和CO的结果没有显著变化。本研究提供了更多关于CO和CONP对分离的大鼠线粒体的 hormetic 效应的信息。
