Liang Qian, Chen Huaguo, Zhou Xin, Deng Qingfang, Hu Enming, Zhao Chao, Gong Xiaojian
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, P. R. China.
Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guiyang, P. R. China.
Electrophoresis. 2017 Apr;38(8):1113-1121. doi: 10.1002/elps.201600515. Epub 2017 Feb 21.
Microwave extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. was investigated in this study, extraction process was first performed in an ultrasonic cleaner, then treated by microwave irradiation. The optimum ultrasonic time of 25 min was selected by single-factor experiments. A response surface methodology has been used to obtain a mathematical model that describes the process and analyzes the significant parameters ethanol concentration 59.92%, liquid to raw materials ratio 21.24 mL/g, microwave radiation time 209.53 s, and microwave power 274.14 w. In these optimum conditions, the yield of flavonoids from P. forrestii (TFPF) could be up to 9.11 ± 0.08%, which was increased by 14.30 and 19.86% compared microwave extraction and ultrasonic extraction, respectively. In vitro suppress hyaluronidase experimentation showed that TFPF purified using polyamide exhibited good anti-hyaluronidase ability with IC value of 1.033 mg/mL, possessing certain anti-antiallergic and potential application prospect in pharmaceutical production of treating inflammation and other related fields.
本研究考察了微波提取结合超声预处理从丽江杠柳中提取黄酮类化合物的方法，提取过程先在超声波清洗器中进行，然后进行微波辐射处理。通过单因素实验选择了25分钟的最佳超声时间。采用响应面法获得了描述该过程的数学模型，并分析了显著参数：乙醇浓度59.92%、液料比21.24 mL/g、微波辐射时间209.53 s和微波功率274.14 w。在这些最佳条件下，丽江杠柳黄酮（TFPF）的得率可达9.11±0.08%，分别比微波提取和超声提取提高了14.30%和19.86%。体外抑制透明质酸酶实验表明，用聚酰胺纯化的TFPF具有良好的抗透明质酸酶能力，IC值为1.033 mg/mL，在治疗炎症等相关领域的药物生产中具有一定的抗过敏及潜在应用前景。
