Sakakibara Akiko, Sakakibara Shunsuke, Kusumoto Junya, Takeda Daisuke, Hasegawa Takumi, Akashi Masaya, Minamikawa Tsutomu, Hashikawa Kazunobu, Terashi Hiroto, Komori Takahide
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Department of Plastic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
PLoS One. 2017 Jan 12;12(1):e0169723. doi: 10.1371/journal.pone.0169723. eCollection 2017.
Transient receptor potential cation channel (subfamily V, members 1-4) (TRPV1-4) are expressed in skin and neurons and activated by external stimuli in normal mucosae of all oral cavity sites. The oral cavity is exposed to various stimuli, including temperature, mechanical stimuli, chemical substances, and changes in pH, and, notably, the risk factors for oncogenic transformation in oral squamous epithelium are the same as the external stimuli received by TRPV1-4 receptors. Hence, we examined the relationship between oral squamous cell carcinoma (SCC) and TRPV1-4 expression.
Oral SCC patients (n = 37) who underwent surgical resection were included in this study. We investigated the expression of TRPV1-4 by immunohistochemical staining and quantification of TRPV1-4 mRNA in human oral mucosa. In addition, we compared the TRPV1-4 levels in mucosa from patients with SCC to those in normal oral mucosa.
The receptors were expressed in oral mucosa at all sites (tongue, buccal mucosa, gingiva, and oral floor) and the expression was stronger in epithelia from patients with SCC than in normal epithelia. Furthermore, alcohol consumption and tobacco use were strongly associated with the occurrence of oral cancer and were found to have a remarkable influence on TRPV1-4 receptor expression in normal oral mucosa. In particular, patients with a history of alcohol consumption demonstrated significantly higher expression levels.
Various external stimuli may influence the behavior of cancer cells. Overexpression of TRPV1-4 is likely to be a factor in enhanced sensitivity to external stimuli. These findings could contribute to the establishment of novel strategies for cancer therapy or prevention.
瞬时受体电位阳离子通道（V亚家族，成员1 - 4）（TRPV1 - 4）在皮肤和神经元中表达，并在口腔所有部位的正常黏膜中被外部刺激激活。口腔会受到各种刺激，包括温度、机械刺激、化学物质和pH值变化，值得注意的是，口腔鳞状上皮致癌转化的危险因素与TRPV1 - 4受体所接受的外部刺激相同。因此，我们研究了口腔鳞状细胞癌（SCC）与TRPV1 - 4表达之间的关系。
本研究纳入了37例行手术切除的口腔SCC患者。我们通过免疫组织化学染色和定量检测人口腔黏膜中TRPV1 - 4 mRNA来研究TRPV1 - 4的表达。此外，我们比较了SCC患者黏膜与正常口腔黏膜中TRPV1 - 4的水平。
这些受体在口腔所有部位（舌、颊黏膜、牙龈和口底）的黏膜中均有表达，且SCC患者上皮中的表达比正常上皮更强。此外，饮酒和吸烟与口腔癌的发生密切相关，并且发现它们对正常口腔黏膜中TRPV1 - 4受体的表达有显著影响。特别是，有饮酒史的患者表现出明显更高的表达水平。
各种外部刺激可能影响癌细胞的行为。TRPV1 - 4的过表达可能是对外部刺激敏感性增强的一个因素。这些发现可能有助于建立新的癌症治疗或预防策略。
