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微小RNA-214-5p靶向ROCK1并抑制人骨肉瘤细胞的增殖和侵袭。

miR-214-5p Targets ROCK1 and Suppresses Proliferation and Invasion of Human Osteosarcoma Cells.

作者信息

Zhang Minglei, Wang Dapeng, Zhu Tongtong, Yin Ruofeng

机构信息

Department of Orthopaedics, ChinaJapan Union Hospital, Jilin UniversityChangchunP.R. China.

Department of Orthopaedics, Si-ping Central HospitalSipingP.R. China.

出版信息

Oncol Res. 2017 Jan 2;25(1):75-81. doi: 10.3727/096504016X14719078133401.

DOI:10.3727/096504016X14719078133401
PMID:28081735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7840756/
Abstract

MicroRNAs (miRNAs) are small conserved RNAs regulating specific target genes in posttranscriptional levels. They have been involved in multiple processes of tumor progression, including cell proliferation. miR-214-5p (also miR-214*) is a newly identified miRNA, and its functions are largely unknown. In this study, we explore the role of miR-214-5p in the proliferation and invasion of human osteosarcoma (OS) cells. The results showed that miR-214-5p was sharply reduced in OS tissues and cell lines, compared with normal tissues and cell lines. In addition, the miR-214-5p mimic greatly increased the miR-214-5p level and significantly decreased the proliferation and invasion of HOS and G293 OS cells. In contrast, the miR-214-5p inhibitor had a completely opposite effect on the miR-214-5p level, cell proliferation, and cell invasion. Moreover, bioinformatics and luciferase reporter gene assays confirmed that miR-1908 targeted the mRNA 3'-UTR region of ROCK1, a characterized tumor promoter in OS. In conclusion, miR-214-5p was identified as a new tumor suppressor, which directly targeted ROCK1 and suppressed proliferation of human OS cells.

摘要

微小RNA（miRNA）是一类保守的小RNA，可在转录后水平调控特定靶基因。它们参与了肿瘤进展的多个过程，包括细胞增殖。miR-214-5p（也称为miR-214*）是一种新发现的miRNA，其功能在很大程度上尚不清楚。在本研究中，我们探讨了miR-214-5p在人骨肉瘤（OS）细胞增殖和侵袭中的作用。结果显示，与正常组织和细胞系相比，miR-214-5p在OS组织和细胞系中显著降低。此外，miR-214-5p模拟物极大地提高了miR-214-5p水平，并显著降低了HOS和G293 OS细胞的增殖和侵袭。相反，miR-214-5p抑制剂对miR-214-5p水平、细胞增殖和细胞侵袭具有完全相反的作用。此外，生物信息学和荧光素酶报告基因检测证实，miR-1908靶向ROCK1的mRNA 3'-UTR区域，ROCK1是OS中一种已明确的肿瘤促进因子。总之，miR-214-5p被鉴定为一种新的肿瘤抑制因子，它直接靶向ROCK1并抑制人OS细胞的增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/1facf43fccd1/OR-25-075-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/6e14d2169a9f/OR-25-075-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/32e9e2413fc6/OR-25-075-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/c8802e5f6ba2/OR-25-075-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/1facf43fccd1/OR-25-075-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/6e14d2169a9f/OR-25-075-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/32e9e2413fc6/OR-25-075-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/c8802e5f6ba2/OR-25-075-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf51/7840756/1facf43fccd1/OR-25-075-g004.jpg

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