Zhang Minglei, Wang Dapeng, Zhu Tongtong, Yin Ruofeng
Department of Orthopaedics, ChinaJapan Union Hospital, Jilin UniversityChangchunP.R. China.
Department of Orthopaedics, Si-ping Central HospitalSipingP.R. China.
Oncol Res. 2017 Jan 2;25(1):83-91. doi: 10.3727/096504016X14719078133447.
RASSF4, a member of the RASSF family, is broadly expressed in normal tissues but often inactivated in human cancers. Despite various studies on RASSF4, its role in osteosarcoma remains unclear. Therefore, in this study, we investigated the effects of RASSF4 expression on osteosarcoma cells and explored the underlying mechanism. The results of our study showed that RASSF4 was lowly expressed in osteosarcoma tissues and cells. RASSF4 overexpression significantly inhibited proliferation, migration, and invasion as well as the EMT process in osteosarcoma cells. Meanwhile, we found that RASSF4 overexpression markedly decreased the protein expression of β-catenin, cyclin D1, and c-Myc in osteosarcoma cells. In conclusion, our findings showed that RASSF4 overexpression inhibits proliferation, invasion, EMT, and Wnt signaling pathway in osteosarcoma cells. Thus, RASSF4 may be considered a novel target for osteosarcoma treatment.
RASSF4是RASSF家族的成员之一，在正常组织中广泛表达，但在人类癌症中常被灭活。尽管对RASSF4进行了各种研究，但其在骨肉瘤中的作用仍不清楚。因此，在本研究中，我们研究了RASSF4表达对骨肉瘤细胞的影响，并探讨了其潜在机制。我们的研究结果表明，RASSF4在骨肉瘤组织和细胞中低表达。RASSF4过表达显著抑制骨肉瘤细胞的增殖、迁移和侵袭以及EMT过程。同时，我们发现RASSF4过表达显著降低了骨肉瘤细胞中β-连环蛋白、细胞周期蛋白D1和c-Myc的蛋白表达。总之，我们的研究结果表明，RASSF4过表达抑制骨肉瘤细胞的增殖、侵袭、EMT和Wnt信号通路。因此，RASSF4可能被认为是骨肉瘤治疗的新靶点。
