Zarabadi Atefeh S, Pawliszyn Janusz, Hajialamdari Mojtaba
Department of Chemistry, University of Waterloo, Waterloo, Ontario, N2 l 3G1, Canada.
Department of Chemistry, University of Waterloo, Waterloo, Ontario, N2 l 3G1, Canada.
J Chromatogr A. 2017 Feb 10;1484:93-97. doi: 10.1016/j.chroma.2017.01.007. Epub 2017 Jan 3.
The concentration gradient detection method based on the Schlieren optics employed for electrophoresis analyses by extending the technology to a multi-channel system using a prototyped microfluidic chip (thinXXS Micro-technology, Germany). The results prove that coupling a chip-based microfluidic device with Schlieren detection is an appropriate approach to improve the electrophoretic separations. The effects of channel's geometry and dimension were investigated by conducting the experiments in channels with different cross sectional areas. Fast kinetic data acquisition of the charge-coupled device (CCD) camera facilitated recording of a time sequence of optical images, demonstrating the potential of the CCD camera as a powerful tool for studying dynamic processes such as diffusion. Diffusion coefficients of sample proteins were measured under static and dynamic conditions, where the static mode demonstrated more accurate results. Furthermore, the Fourier transformation was employed to improve the Schlieren images for quantitative analysis of the diffusion coefficient measurement.
基于纹影光学的浓度梯度检测方法通过使用原型微流控芯片（德国thinXXS微技术公司）将该技术扩展到多通道系统，用于电泳分析。结果证明，将基于芯片的微流控装置与纹影检测相结合是改善电泳分离的一种合适方法。通过在具有不同横截面积的通道中进行实验，研究了通道几何形状和尺寸的影响。电荷耦合器件（CCD）相机的快速动力学数据采集有助于记录光学图像的时间序列，证明了CCD相机作为研究扩散等动态过程的强大工具的潜力。在静态和动态条件下测量了样品蛋白质的扩散系数，其中静态模式显示出更准确的结果。此外，采用傅里叶变换来改善纹影图像，以便对扩散系数测量进行定量分析。
