Ghiaseddin Ali, Pouri Hossein, Soleimani Masoud, Vasheghani-Farahani Ebrahim, Ahmadi Tafti Hossein, Hashemi-Najafabadi Sameereh
Biomedical Engineering Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Hematology Group, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Biochem Biophys Res Commun. 2017 Mar 4;484(2):225-230. doi: 10.1016/j.bbrc.2017.01.029. Epub 2017 Jan 9.
Since the leading cause of death are cardiac diseases, engineered heart tissue (EHT) is one of the most appealing topics defined in tissue engineering and regenerative medicine fields. The importance of EHT is not only for heart regeneration but also for in vitro developing of cardiology. Cardiomyocytes could grow and commit more naturally in their microenvironment rather than traditional cultivation. Thus, this research tried to develop a set up on-a-chip to produce EHT based on chitosan hydrogel. Micro-bioreactor was hydrodynamically designed and simulated by COMSOL and produced via soft lithography process. Chitosan hydrogel was also prepared, adjusted, and assessed by XRD, FTIR and also its degradation rate and swelling ratio were determined. Finally, hydrogels in which mice cardiac progenitor cells (CPC) were loaded were injected into the micro-device chambers and cultured. Each EHT in every chamber was evaluated separately. Prepared EHTs showed promising results that expanded in them CPCs and work as an integrated syncytium. High cell density culture was the main accomplishment of this study.
由于主要死因是心脏疾病，工程化心脏组织（EHT）是组织工程和再生医学领域中最具吸引力的课题之一。EHT的重要性不仅在于心脏再生，还在于心脏病学的体外发展。心肌细胞在其微环境中比传统培养方式能够更自然地生长和分化。因此，本研究试图开发一种基于壳聚糖水凝胶的芯片装置来生产EHT。微生物反应器通过COMSOL进行流体动力学设计和模拟，并通过软光刻工艺制造。壳聚糖水凝胶也进行了制备、调整，并通过XRD、FTIR进行评估，同时测定了其降解速率和溶胀率。最后，将负载有小鼠心脏祖细胞（CPC）的水凝胶注入微器件腔室中进行培养。每个腔室中的每个EHT都分别进行了评估。制备的EHT显示出有前景的结果，其中的CPC得以扩增并作为一个整合的合胞体发挥作用。高细胞密度培养是本研究的主要成果。
