Rami Lila, Malaise Sebastien, Delmond Samantha, Fricain Jean-Christophe, Siadous Robin, Schlaubitz Silke, Laurichesse Eric, Amédée Joëlle, Montembault Alexandra, David Laurent, Bordenave Laurence
Université de Bordeaux, Bordeaux, 33000, France; Inserm U1026, Bioingénierie Tissulaire, Bordeaux, 33000, France.
J Biomed Mater Res A. 2014 Oct;102(10):3666-76. doi: 10.1002/jbm.a.35035. Epub 2013 Nov 30.
Polysaccharide-based hydrogels are remarkable materials for the development of tissue engineering strategies as they meet several critical requirements for such applications and they may partly mimic the extracellular matrix. Chitosan is widely envisioned as hydrogel in biomedical fields for its bioresorbability, biocompatibility, and fungistatic and bacteriostatic properties. In this study, we report that the modulation of the polymer concentration, the degree of acetylation, the gelation processes [or neutralization routes (NR)] in the preparation of different chitosan-based hydrogels lead to substantially and significantly different biological responses. We show that it is possible to tune the physicochemical characteristics, mechanical properties, and biological responses of such matrices. Physical hydrogels prepared from highly acetylated chitosan were softer, degraded quickly in vivo, and were not suitable for in vitro culture of human mesenchymal stem and progenitor derived endothelial cells. In contrast, for a same chitosan concentration and obtained by the same processing route, a low degree of acetylation chitosan hydrogel provided a more elastic material, better cell adhesion on its surface and tissue regeneration, and restored tissue neo-vascularization as well. This work offers promising and innovative perspectives for the design of hydrogel materials with tunable properties for tissue engineering and regenerative medicine.
基于多糖的水凝胶是用于组织工程策略开发的卓越材料，因为它们满足此类应用的几个关键要求，并且可以部分模拟细胞外基质。壳聚糖因其生物可吸收性、生物相容性以及抑菌和杀菌特性，在生物医学领域被广泛设想为水凝胶。在本研究中，我们报告了在制备不同的基于壳聚糖的水凝胶时，聚合物浓度、乙酰化程度、凝胶化过程[或中和途径（NR）]的调节会导致显著且明显不同的生物学反应。我们表明，可以调节此类基质的物理化学特性、机械性能和生物学反应。由高度乙酰化壳聚糖制备的物理水凝胶更柔软，在体内快速降解，不适合人骨髓间充质干细胞和祖细胞来源的内皮细胞的体外培养。相比之下，对于相同的壳聚糖浓度并通过相同的加工路线获得的低乙酰化程度壳聚糖水凝胶提供了更具弹性的材料，其表面具有更好的细胞粘附性和组织再生能力，并且还恢复了组织新血管形成。这项工作为设计具有可调特性的水凝胶材料用于组织工程和再生医学提供了有前景和创新性的观点。
