Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Malaga, Spain.
Center for Plant Biotechnology and Genomic (UPM-INIA), Madrid, Spain.
Sci Rep. 2017 Jan 13;7:40449. doi: 10.1038/srep40449.
Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a proliferative CD4 T-cell response. These results demonstrate that Pru p 3-specific anaphylaxis can be generated after nasal sensitisation to Pru p 3 in combination with LPS. This is a promising model for evaluating food allergy immunotherapies.
普魯帕 3 是地中海地區桃樹的主要過敏原。它常引起嚴重的反應，限制了人體對其的研究，因此需要動物模型來研究涉及的免疫機制。然而，目前還沒有普魯帕 3 的過敏反應模型。本研究旨在通過用普魯帕 3 結合脂多糖（LPS）作為佐劑鼻內致敏小鼠來建立桃樹過敏的模型。四組小鼠分別接受以下處理：未處理；用普魯帕 3 處理；用 LPS 處理；用普魯帕 3+LPS 處理。致敏後，用普魯帕 3 腹腔內攻擊小鼠，並評估體內和體外參數。只有普魯帕 3+LPS 組的小鼠出現過敏症狀，包括體溫下降。測定體外參數顯示 Th2 反應增加，表現為普魯帕 3 特異性 IgE 和 IgG1 增加。此外，在細胞水平上，我們發現普魯帕 3 特異性 IgE 和 IgG1 分泌細胞以及增殖的 CD4 T 細胞反應增加。這些結果表明，普魯帕 3 特異性過敏反應可以在鼻內用普魯帕 3 結合 LPS 致敏後產生。這是一種用於評估食物過敏免疫治療的有前途的模型。
