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葵花籽蛋白在小鼠模型中的致敏效力：鉴定比 SFA-8 更具过敏原性的 2S-清蛋白。

Sensitization Potency of Sunflower Seed Protein in a Mouse Model: Identification of 2S-Albumins More Allergenic Than SFA-8.

机构信息

CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS)/Service de Pharmacologie et d'Immunoanalyse, Université Paris-Saclay, Gif-sur-Yvette, France.

Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, Université de Lorraine, Nancy, France.

出版信息

Mol Nutr Food Res. 2021 Sep;65(18):e2100369. doi: 10.1002/mnfr.202100369. Epub 2021 Aug 19.

DOI:10.1002/mnfr.202100369
PMID:34331387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9285957/
Abstract

SCOPE

Food allergy to sunflower seed (SFS) protein is not frequent and only non-specific lipid transfert protein (nsLTP) Hel a 3 is officially recognized as a food allergen. Out of the eleven seed storage 2S-albumins (SESA) detected in SFS, only SFA-8 allergenicity has been investigated so far. The study aimed then to evaluate SFS protein allergenicity and particularly, to compare the sensitization potency of SESA in a mouse model.

METHODS AND RESULTS

The most abundant SESA and nsLTP were isolated from SFS through a combination of chromatographic methods. Purified proteins were then used to measure specific IgG1 and IgE responses in BALB/c mice orally sensitized to different SFS protein isolates. The study, thus, confirmed the allergenicity of SFA-8 and Hel a 3 but mice were also highly sensitized to other SESA such as SESA2-1 or SESA20-2. Furthermore, competitive inhibition of IgE-binding revealed that SFA-8 IgE-reactivity was due to cross-reactivity with other SESA. 11S-globulins were weakly immunogenic and were rapidly degraded in an in vitro model of gastroduodenal digestion. In contrast, Hel a 3, SESA2-1 and SFA-8 were more resistant to proteolysis and gastroduodenal digestion did not affect their IgE-reactivity.

CONCLUSIONS

SESA2-1 or SESA20-2 were more potent allergens than SFA-8 in this mouse model. Allergenicity of SESA must be now confirmed in SFS-allergic patients.

摘要

范围

葵花籽蛋白（SFS）过敏并不常见，只有非特异性脂质转移蛋白（nsLTP）Hel a 3 被正式认定为食物过敏原。在 SFS 中检测到的十一种种子贮藏 2S-白蛋白（SESA）中，迄今为止仅研究了 SFA-8 的致敏性。因此，本研究旨在评估 SFS 蛋白的致敏性，特别是在小鼠模型中比较 SESA 的致敏潜力。

方法和结果

通过组合色谱方法从 SFS 中分离出最丰富的 SESA 和 nsLTP。然后，使用纯化的蛋白质来测量经不同 SFS 蛋白分离物口服致敏的 BALB/c 小鼠的特异性 IgG1 和 IgE 反应。研究证实了 SFA-8 和 Hel a 3 的致敏性，但小鼠也对其他 SESA（如 SESA2-1 或 SESA20-2）高度敏感。此外，IgE 结合的竞争抑制表明 SFA-8 IgE 反应性是由于与其他 SESA 的交叉反应性所致。11S 球蛋白的免疫原性较弱，在胃十二指肠消化的体外模型中迅速降解。相比之下，Hel a 3、SESA2-1 和 SFA-8 对蛋白水解的抵抗力更强，胃十二指肠消化不会影响它们的 IgE 反应性。

结论

在该小鼠模型中，SESA2-1 或 SESA20-2 比 SFA-8 更具致敏性。SESA 的致敏性现在必须在 SFS 过敏患者中得到证实。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9031/9285957/8bc2eb98ea9e/MNFR-65-0-g007.jpg
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