Hong Ji Yeon, Choi Yong Hee, Roh Yoon Jin, Lee Mi-Kyung, Zouboulis Christos C, Park Kui Young
Department of Dermatology, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Department of Laboratory Medicine, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Front Med (Lausanne). 2025 Jan 29;12:1518382. doi: 10.3389/fmed.2025.1518382. eCollection 2025.
Afzelin, a flavonoid (kaempferol 3-O-α-L-rhamnopyranoside) isolated from , is known for its potent anti-inflammatory properties. However, its effects on the molecular aspects of inflammation and lipogenesis in SZ95 sebocytes has not been investigated.
This study aimed to (i) investigate inflammatory and sebum secretion changes when a -treated immortalized human sebocyte cell line (SZ95) is exposed to particulate matter (PM) and (ii) examine the effects of afzelin on these.
To investigate the effect of afzelin on PM- and -treated SZ95 sebocytes, we injected heat-killed into SZ95 cells to induce acne-like status. Thereafter, the SZ95 sebocytes were treated with PM and subsequently with afzelin. The gene expression profile was determined using real-time polymerase chain reaction analysis, and protein expression was confirmed via western blotting and immunofluorescence. Intracellular lipid droplet formation was investigated using Nile Red O staining.
PM treatment upregulated the mRNA and protein expression levels of inflammatory cytokine and lipogenic genes in -treated SZ95 sebocytes. Furthermore, intracellular lipid-droplet accumulation increased when -stimulated SZ95 cells were exposed to PM. Interestingly, the upregulated inflammatory and lipogenic gene expression induced by and PM was attenuated by afzelin treatment.
This study's findings indicate that PM potentially aggravates acne by acting on both inflammation and sebum secretion. They also reveal afzelin's ability to suppress these phenomena by not only suppressing inflammatory cytokine expression but also inhibiting sebogenesis. These findings confirm afzelin's potential therapeutic role in improving PM-exacerbated acne.
从[具体来源未给出]中分离出的黄酮类化合物（山奈酚3 - O -α - L - 鼠李糖苷）——桤木酮，以其强大的抗炎特性而闻名。然而，其对SZ95皮脂腺细胞炎症和脂肪生成分子层面的影响尚未得到研究。
本研究旨在（i）调查经[具体处理未明确]处理的永生化人皮脂腺细胞系（SZ95）暴露于颗粒物（PM）时的炎症和皮脂分泌变化，以及（ii）研究桤木酮对这些变化的影响。
为了研究桤木酮对经PM和[具体处理未明确]处理的SZ95皮脂腺细胞的影响，我们将热灭活的[具体物质未明确]注射到SZ95细胞中以诱导类似痤疮的状态。此后，用PM处理SZ95皮脂腺细胞，随后用桤木酮处理。使用实时聚合酶链反应分析确定基因表达谱，并通过蛋白质印迹和免疫荧光确认蛋白质表达。使用尼罗红O染色研究细胞内脂滴形成。
PM处理上调了经[具体处理未明确]处理的SZ95皮脂腺细胞中炎性细胞因子和脂肪生成基因的mRNA和蛋白质表达水平。此外，当经[具体刺激未明确]刺激的SZ95细胞暴露于PM时，细胞内脂滴积累增加。有趣的是，桤木酮处理减弱了由[具体处理未明确]和PM诱导的炎性和脂肪生成基因表达上调。
本研究结果表明，PM可能通过作用于炎症和皮脂分泌来加重痤疮。它们还揭示了桤木酮不仅通过抑制炎性细胞因子表达，还通过抑制皮脂生成来抑制这些现象的能力。这些发现证实了桤木酮在改善PM加重的痤疮方面的潜在治疗作用。
