Yu Siran, Zhao Zhehao, Sun Liming, Li Ping
Research Center for Translational Medicine at Shanghai East Hospital, School of Life Science and Technology, Tongji University , Shanghai 200092, PR China.
Petrochemical Research Institute, PetroChina Company Limited , Beijing 102206, PR China.
J Agric Food Chem. 2017 Feb 15;65(6):1220-1228. doi: 10.1021/acs.jafc.6b05002. Epub 2017 Feb 3.
The discovery of microRNAs encapsulated in milk-derived exosomes has revealed stability under extreme conditions reflecting the protection of membranes. We attempted to determine the variations in nanoparticles derived from milk after fermentation, and provide evidence to determine the effects of these exosomes on cells with potential bioactivity. Using scanning electron microscopy and dynamic light scattering, we compared the morphology and particle size distribution of exosomes from yogurt fermented with three different combinations of strains with those from raw milk. The protein content of the exosome was significantly reduced in fermented milk. The cycle threshold showed that the expression of miR-29b and miR-21 was relatively high in raw milk, indicating a loss of microRNA after fermentation. Milk-derived exosomes could promote cell growth and activate the mitogen-activated protein kinase pathway. These findings demonstrated biological functions in milk exosomes and provided new insight into the nutrient composition of dairy products.
在源自牛奶的外泌体中发现的微小RNA，显示出在极端条件下的稳定性，这反映了膜的保护作用。我们试图确定发酵后源自牛奶的纳米颗粒的变化，并提供证据来确定这些外泌体对具有潜在生物活性的细胞的影响。使用扫描电子显微镜和动态光散射，我们比较了用三种不同菌株组合发酵的酸奶中的外泌体与原料奶中的外泌体的形态和粒径分布。发酵乳中外泌体的蛋白质含量显著降低。循环阈值表明，原料奶中miR-29b和miR-21的表达相对较高，表明发酵后微小RNA有所损失。源自牛奶的外泌体可以促进细胞生长并激活丝裂原活化蛋白激酶途径。这些发现证明了牛奶外泌体的生物学功能，并为乳制品的营养成分提供了新的见解。
