Montell D J, Goodman C S
Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley 94720.
J Cell Biol. 1989 Nov;109(5):2441-53. doi: 10.1083/jcb.109.5.2441.
In a previous study, we described the cloning of the genes encoding the three subunits of Drosophila laminin, a substrate adhesion molecule, and the cDNA sequence of the B1 subunit (Montell and Goodman, 1988). This analysis revealed the similarity of Drosophila laminin with the mouse and human complexes in subunit composition, domain structure, and amino acid sequence. In this paper, we report the deduced amino acid sequence of the B2 subunit. We then describe the expression and tissue distribution of the three subunits of laminin during Drosophila embryogenesis using both in situ hybridization and immunolocalization techniques, with particular emphasis on its expression in and around the developing nervous system.
在之前的一项研究中，我们描述了果蝇层粘连蛋白（一种底物粘附分子）三个亚基编码基因的克隆以及B1亚基的cDNA序列（蒙特尔和古德曼，1988年）。该分析揭示了果蝇层粘连蛋白在亚基组成、结构域结构和氨基酸序列方面与小鼠及人类复合物的相似性。在本文中，我们报告了B2亚基推导的氨基酸序列。然后，我们使用原位杂交和免疫定位技术描述了果蝇胚胎发育过程中层粘连蛋白三个亚基的表达及组织分布，特别强调了其在发育中的神经系统及其周围的表达情况。
