Mitchison T J, Sedat J
Dev Biol. 1983 Sep;99(1):261-4. doi: 10.1016/0012-1606(83)90275-0.
Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations.
本文描述了从轻度固定的果蝇胚胎中批量去除卵黄膜的方法。所得胚胎保持了良好的结构完整性,现在适用于各种免疫细胞化学和生化特征分析。