Ito Y, Oka H, Slemp J L
Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
J Chromatogr. 1989 Jul 28;475:219-27. doi: 10.1016/s0021-9673(01)89677-1.
A compact desktop model of a high-speed counter-current chromatograph holds three identical multilayer coils in the symmetrical positions around the rotary frame to maintain perfect balance of the centrifuge system without the use of a counterweight. These multilayer coils are connected in series to make up a total capacity of 400 ml while the unique gear arrangement on the rotary frame establishes a twist-free mechanism of the flow tubes so that continuous elution can be performed without the use of rotary seal. The high performance of the present system was successfully demonstrated in separations of 10-250 mg of 2,4-dinitrophenyl amino acid mixtures in a two-phase solvent system composed of chloroform-acetic acid-0.1 M hydrochloric acid (2:2:1, v/v/v).
一种紧凑型台式高速逆流色谱仪，在旋转框架周围的对称位置持有三个相同的多层线圈，无需使用配重即可保持离心系统的完美平衡。这些多层线圈串联连接，总容量为400毫升，而旋转框架上独特的齿轮装置建立了流管的无扭转机制，从而无需使用旋转密封即可进行连续洗脱。在由氯仿 - 乙酸 - 0.1M盐酸（2:2:1，v/v/v）组成的两相溶剂系统中，对10 - 250毫克的2,4 - 二硝基苯基氨基酸混合物进行分离，成功证明了本系统的高性能。
