De Oliveira Fabienne, Mourez Thomas, Vessiere Aurélia, Ngoupo Paul-Alain, Alessandri-Gradt Elodie, Simon François, Rousset Dominique, Plantier Jean-Christophe
GRAM EA2656, Université de Rouen, Rouen, France.
Laboratoire de Virologie, Laboratoire associé au Centre National de Référence du VIH, CHU Charles Nicolle, 76031, Rouen Cedex, France.
Retrovirology. 2017 Jan 13;14(1):1. doi: 10.1186/s12977-016-0324-3.
Due to the prevalence of HIV-1 group M and the endemicity of HIV-1 group O infections in Cameroon, patients may be infected with both viruses and/or with HIV-1/MO recombinant forms. Such atypical infections may be deleterious in terms of diagnosis and therapeutic management due to the high divergence of HIV-1/O. The aim of this study was to identify prospectively such atypical infections in Cameroon.
Based on serological screening by env-V3 serotyping and a molecular strategy using group-specific (RT)-PCRs, we identified 10 Cameroonian patients harboring three different profiles of infection: (1) 4 HIV-1/M + O dual infections without evidence of recombinant; (2) 5 recombinants associated with one or both parental strains; and (3) 1 new recombinant form without parental strains.
This work highlights the dynamic co-evolution of these two HIV groups in Cameroon that could lead to the emergence of a circulating recombinant form MO, and the need for accurate identification of such atypical infections for precise diagnosis, virological monitoring and therapeutic management with adapted tools.
由于喀麦隆存在HIV-1 M组的流行以及HIV-1 O组感染的地方性流行，患者可能同时感染这两种病毒和/或感染HIV-1/MO重组形式。由于HIV-1/O的高度差异，这种非典型感染在诊断和治疗管理方面可能是有害的。本研究的目的是前瞻性地识别喀麦隆的此类非典型感染。
基于env-V3血清学分型的血清学筛查以及使用组特异性（RT）-PCR的分子策略，我们鉴定出10名喀麦隆患者，他们具有三种不同的感染情况：（1）4例HIV-1/M + O双重感染，无重组证据；（2）5例与一种或两种亲本毒株相关的重组体；（3）1例无亲本毒株的新型重组形式。
这项工作突出了喀麦隆这两种HIV组的动态共同进化，这可能导致循环重组形式MO的出现，以及需要使用合适的工具准确识别此类非典型感染以进行精确诊断、病毒学监测和治疗管理。
