Ohishi Takayuki, Aoki Kotaro, Ishii Yoshikazu, Usui Masaru, Tamura Yutaka, Kawanishi Michiko, Ohnishi Kenji, Tateda Kazuhiro
Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan; Department of Infection Control and Prevention, Osaki Citizen Hospital, Miyagi, Japan.
Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan.
J Infect Chemother. 2017 Mar;23(3):165-172. doi: 10.1016/j.jiac.2016.11.011. Epub 2017 Jan 10.
In this research, we analyzed the main sequence types (ST) and ST complexes of human- and chicken-derived isolates of Campylobacter jejuni in Japan by using multilocus sequence typing (MLST). We also analyzed lipooligosaccharide biosynthesis locus classes (LOS locus classes) and the numbers of isolates carrying genes coding resistance factors against various antibiotics, and observed their relationships. ST-21 complex was the main ST complex in isolates from humans (n = 38) and chickens (n = 25). None of the isolates showed resistance to imipenem, chloramphenicol, or erythromycin. Few isolates were resistant to ampicillin and streptomycin (1.3%-15%), whereas many showed resistance to tetracycline, ciprofloxacin, and nalidixic acid (38%-48%). Among the ST-21 complex isolates, ST4526 was detected at a very high rate. Those isolates showed resistance to tetracycline and ciprofloxacin, and were susceptible to ampicillin. Among the chicken-derived isolates, 37 of the 38 isolates that showed resistance to ciprofloxacin and nalidixic acid had threonine to isoleucine amino acid substitution in GyrA at codon 86 (T86I). Among the human-derived isolates, 17 of the 47 isolates that showed resistance to ciprofloxacin and 16 of the 48 isolates that showed resistance to nalidixic acid did not have T86I amino acid mutations in GyrA. The human-derived ST-21 complex isolates were classified into LOS locus classes A, B, C, D, and E. The chicken-derived ST-21 complex isolates, with the exception of one isolate, were all classified into LOS locus classes C and D. Among chicken-derived isolates, the most prevalent was ST51 (ST-443 complex) (10 isolates) and all of those were LOS locus class E.
在本研究中，我们通过多位点序列分型（MLST）分析了日本空肠弯曲菌人源和鸡源分离株的主要序列类型（ST）及ST复合体。我们还分析了脂寡糖生物合成基因座类别（LOS基因座类别）以及携带各种抗生素抗性因子编码基因的分离株数量，并观察了它们之间的关系。ST-21复合体是人类（n = 38）和鸡（n = 25）分离株中的主要ST复合体。所有分离株对亚胺培南、氯霉素或红霉素均无抗性。少数分离株对氨苄西林和链霉素有抗性（1.3%-15%），而许多分离株对四环素、环丙沙星和萘啶酸有抗性（38%-48%）。在ST-21复合体分离株中，ST4526的检出率非常高。这些分离株对四环素和环丙沙星有抗性，对氨苄西林敏感。在鸡源分离株中，38株对环丙沙星和萘啶酸有抗性的分离株中有37株在GyrA的第86位密码子处发生了苏氨酸到异亮氨酸的氨基酸替换（T86I）。在人源分离株中，47株对环丙沙星有抗性的分离株中有17株以及48株对萘啶酸有抗性的分离株中有16株在GyrA中没有T86I氨基酸突变。人源ST-21复合体分离株被分为LOS基因座类别A、B、C、D和E。鸡源ST-21复合体分离株，除一株外，均被分为LOS基因座类别C和D。在鸡源分离株中，最常见的是ST51（ST-443复合体）（10株），所有这些分离株均为LOS基因座类别E。
