Univ. Paris-Est, ICMPE (UMR7182), CNRS, UPEC, Thiais 94320, France; Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France; Medical University of Gdansk, Department of Toxicology, 107 Hallera Street, 80-416 Gdansk, Poland.
Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France.
Anal Chim Acta. 2017 Feb 22;955:1-26. doi: 10.1016/j.aca.2016.12.017. Epub 2016 Dec 31.
Determination of proteins and peptides is among the main challenges of today's bioanalytical chemistry. The application of microchip technology in this field is an exhaustively developed concept that aims to create integrated and fully automated analytical devices able to quantify or detect one or several proteins from a complex matrix. Selective extraction and preconcentration of targeted proteins and peptides especially from biological fluids is of the highest importance for a successful realization of these microsystems. Incorporation of solid structures or supports is a convenient solution employed to face these demands. This review presents a critical view on the latest achievements in sample processing techniques for protein determination using solid supports in microfluidics. The study covers the period from 2006 to 2015 and focuses mainly on the strategies based on microbeads, monolithic materials and membranes. Less common approaches are also briefly discussed. The reviewed literature suggests future trends which are discussed in the concluding remarks.
蛋白质和肽的测定是当今生物分析化学的主要挑战之一。微芯片技术在该领域的应用是一个经过深入开发的概念，旨在创建集成和全自动分析设备，能够从复杂基质中定量或检测一种或多种蛋白质。从生物流体中特别选择性地提取和预浓缩目标蛋白质和肽对于这些微系统的成功实现至关重要。固体结构或载体的结合是解决这些需求的一种便利解决方案。本文对微流控中使用固体载体进行蛋白质测定的最新样品处理技术的最新成果进行了批判性评价。本研究涵盖了 2006 年至 2015 年期间，主要集中在基于微珠、整体材料和膜的策略上。也简要讨论了不太常见的方法。综述文献提出了未来的发展趋势，在结束语中进行了讨论。
