Max Planck Institute for Terrestrial Microbiology, Dept. Organismic Interactions, 35043 Marburg, Germany.
Max Planck Institute for Terrestrial Microbiology, Dept. Organismic Interactions, 35043 Marburg, Germany.
Fungal Genet Biol. 2018 Mar;112:21-30. doi: 10.1016/j.fgb.2016.12.003. Epub 2017 Jan 6.
In the ten years since the genome sequence of the basidiomycete corn smut fungus Ustilago maydis was published, additional genomes of smut species infecting different hosts became available. In addition, the genomes of related Malassezia species causing skin diseases and of Pseudozyma species not known to infect plants were determined. As secreted proteins are critical virulence determinants in U. maydis we compare here the secretomes of 12 basidiomycete species to gain information about their composition and conservation. For this we classify secreted proteins into those with and without domains using InterPro scans. Homology among proteins is inferred by building clusters based on pairwise similarities and cluster presence is then assessed in the different species. We detect in particular a strong correspondence between the secretomes of Pseudozyma species and plant infecting smuts. Furthermore, we identify a high proportion of secreted proteins to be part of gene families and present an advancement of the CRISPR-Cas9 technology for simultaneous disruption of multiple genes in U. maydis using five genes of the eff1 family as example.
在担子菌玉米黑粉菌 Ustilago maydis 的基因组序列公布后的十年中，更多感染不同宿主的黑粉菌物种的基因组也相继公布。此外，还确定了与引起皮肤疾病的相关马拉色菌属物种以及尚未知是否感染植物的 Pseudozyma 物种的基因组。由于分泌蛋白是 U. maydis 中关键的毒力决定因素，我们在这里比较了 12 种担子菌的分泌组，以获取有关其组成和保守性的信息。为此，我们使用 InterPro 扫描将分泌蛋白分为具有和不具有结构域的蛋白。通过基于两两相似度构建聚类来推断蛋白质之间的同源性，然后评估不同物种中聚类的存在情况。我们特别发现 Pseudozyma 物种的分泌组与感染植物的黑粉菌之间存在很强的对应关系。此外，我们还发现很大一部分分泌蛋白是基因家族的一部分，并展示了一种用于同时敲除 U. maydis 中多个基因的 CRISPR-Cas9 技术的进展，以 eff1 家族的 5 个基因为例。
