Soltani El-Khamsa, Cerezuela Rebeca, Charef Noureddine, Mezaache-Aichour Samia, Esteban Maria Angeles, Zerroug Mohamed Mihoub
Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas Sétif 1, Algeria.
Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
Fish Shellfish Immunol. 2017 Mar;62:57-67. doi: 10.1016/j.fsi.2017.01.009. Epub 2017 Jan 9.
Propolis has been used as a medicinal agent for centuries. The chemical composition of four propolis samples collected from four locations of the Sétif region, Algeria, using gas chromatography-mass spectrometry was determined. More than 20 compounds and from 30 to 35 compounds were identified in the aqueous and ethanolic extracts, respectively. Furthermore, the antimicrobial activity of the propolis extracts against two marine pathogenic bacteria was evaluated. Finally, the in vitro effects of propolis on gilthead seabream (Sparus aurata L.) leucocyte activities were measured. The bactericidal activity of ethanolic extracts was very high against Shewanella putrefaciens, average against Photobacterium damselae and very low against Vibrio harveyi. The lowest bactericidal activity was always that found for the aqueous extracts. When the viability of gilthead seabream head-kidney leucocytes was measured after 30 min' incubation with the different extracts, both the ethanolic and aqueous extracts of one of the propolis samples (from Babor) and the aqueous extract of another (from Ain-Abbassa) provoked a significant decrease in cell viability when used at concentrations of 100 and 200 μg ml. Furthermore, significant inhibitory effects were recorded on leucocyte respiratory burst activity when isolated leucocytes where preincubated with the extracts. This effect was dose-dependent in all cases except when extracts from a third propolis sample (from Boutaleb) were used. Our findings suggest that some of Algerian propolis extracts have bactericidal activity against important bacterial pathogens in seabream and significantly modulate in vitro leucocyte activities, confirming their potential as a source of new natural biocides and/or immunomodulators in aquaculture practice.
几个世纪以来，蜂胶一直被用作一种药物。使用气相色谱 - 质谱法测定了从阿尔及利亚塞提夫地区四个地点采集的四个蜂胶样品的化学成分。在水提取物和乙醇提取物中分别鉴定出20多种化合物和30至35种化合物。此外，还评估了蜂胶提取物对两种海洋致病细菌的抗菌活性。最后，测定了蜂胶对金头鲷（Sparus aurata L.）白细胞活性的体外影响。乙醇提取物对腐败希瓦氏菌的杀菌活性非常高，对美人鱼发光杆菌的杀菌活性为中等，对哈维氏弧菌的杀菌活性非常低。水提取物的杀菌活性始终是最低的。当用不同提取物孵育30分钟后测量金头鲷头肾白细胞的活力时，其中一个蜂胶样品（来自巴卜尔）的乙醇提取物和水提取物以及另一个（来自艾因 - 阿巴萨）的水提取物在浓度为100和200μg/ml时均导致细胞活力显著下降。此外，当分离的白细胞与提取物预孵育时，记录到对白细胞呼吸爆发活性有显著抑制作用。除了使用来自第三个蜂胶样品（来自布塔莱布）的提取物时，在所有情况下这种作用都是剂量依赖性的。我们的研究结果表明，一些阿尔及利亚蜂胶提取物对金头鲷中的重要细菌病原体具有杀菌活性，并能显著调节体外白细胞活性，证实了它们作为水产养殖实践中新的天然杀菌剂和/或免疫调节剂来源的潜力。
