From the Department of Medical Laboratory Technology (Bawazir), Faculty of Applied Medical Sciences; and from the Hematology Research Unit (Bawazir), King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Saudi Med J. 2022 Mar;43(3):244-251. doi: 10.15537/smj.2022.43.3.20210680.
To establish the frequency of Dombrock (DO) blood group genotypes in Western Saudi Arabians and to compare the findings with other populations in the 1000 genomes database.
This cross-sectional study was carried out between December 2018 and February 2019. A total of 440 blood samples in ethylenediaminetetraacetic acid tubes were collected from unrelated Saudi Arabian blood donors from Jeddah, Saudi Arabia. Deoxyribonucleic acid was extracted, followed by an allele-specific polymerase chain reaction for DO01 and DO02 alleles (, ). The allele and genotype frequencies were counted and compared to those in other populations using the Chi-squared test with Bonferroni adjustments.
The DO allele frequencies for blood donors from western Saudi Arabia were 0.432 for DO01 and 0.568 for DO02. The DO genotype frequencies were 0.182 for DO01/01, 0.318 for DO02/02, and 0.5 for DO*01/02. The DO genotype frequencies were similar to Europeans, Americans, and South Asians but significantly different from the genotype frequencies of Africans and East Asians reported in the 1000 genomes database.
Dombrock genotype frequencies in the Western Saudi Arabian population were different from Africans and East Asians but not from Europeans, Americans, and South Asians. This study contributes to a genotyped blood donor database and may advance transfusion safety for patients in western Saudi Arabia.
确定沙特阿拉伯西部地区多布罗克（DO）血型基因型的出现频率，并与 1000 基因组数据库中的其他人群进行比较。
本横断面研究于 2018 年 12 月至 2019 年 2 月进行。从沙特阿拉伯吉达的无关沙特献血者采集了 440 份 EDTA 管血样。提取脱氧核糖核酸后，采用等位基因特异性聚合酶链反应检测 DO01 和 DO02 等位基因（，）。计数等位基因和基因型频率，并与其他人群进行比较，使用卡方检验和 Bonferroni 校正。
来自沙特阿拉伯西部的献血者 DO 等位基因频率分别为 DO01 为 0.432，DO02 为 0.568。DO 基因型频率分别为 DO01/01 为 0.182，DO02/02 为 0.318，DO*01/02 为 0.5。DO 基因型频率与欧洲人、美洲人和南亚人相似，但与 1000 基因组数据库中报道的非洲人和东亚人的基因型频率有显著差异。
沙特阿拉伯西部地区多布罗克基因型频率与非洲人和东亚人不同，但与欧洲人、美洲人和南亚人相同。本研究为沙特阿拉伯西部的基因分型献血者数据库做出了贡献，并可能提高该地区患者的输血安全性。
