Merck Endowed Chair for Molecular Biotechnology, Department of Biosciences and Buchmann Institute for Molecular Life Sciences (BMLS), Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt am Main, Germany.
Curr Top Microbiol Immunol. 2017;402:55-79. doi: 10.1007/82_2016_24.
Although the first natural products (NP) from Photorhabdus and Xenorhabdus bacteria have been known now for almost 30 years, a huge variety of new compounds have been identified in the last 5-10 years, mainly due to the application of modern mass spectrometry. Additionally, application of molecular methods that allow the activation of NP production in several different strains as well as efficient heterologous expression methods have led to the production and validation of many new compounds. In this chapter we discuss the benefit of using Photorhabdus as a model system for microbial chemical ecology. We also examine non-ribosomal peptide synthetases as the most important pathway for NP production. Finally, we discuss the origin and function of all currently known NPs and the development of the molecular and chemical tools used to identify these NPs faster.
虽然 Photorhabdus 和 Xenorhabdus 细菌的第一批天然产物 (NP) 已经为人所知近 30 年，但在过去的 5-10 年中，由于现代质谱技术的应用，已经鉴定出了大量新的化合物。此外，应用允许在几种不同菌株中激活 NP 产生的分子方法以及有效的异源表达方法，导致了许多新化合物的产生和验证。在本章中，我们讨论了使用 Photorhabdus 作为微生物化学生态学模型系统的好处。我们还研究了非核糖体肽合成酶作为 NP 产生的最重要途径。最后，我们讨论了所有目前已知的 NP 的起源和功能，以及开发用于更快识别这些 NP 的分子和化学工具的发展。
