Department of Entomology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI 53706, USA.
Biotechnology Center, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI 53706, USA.
Sci Adv. 2019 Jan 16;5(1):eaau6141. doi: 10.1126/sciadv.aau6141. eCollection 2019 Jan.
Antibiotic and insecticidal bioactivities of the extracellular secondary metabolites produced by entomopathogenic bacteria belonging to genus have been identified; however, their novel applications such as mosquito feeding-deterrence have not been reported. Here, we show that a mixture of compounds isolated from in vitro cultures exhibits potent feeding-deterrent activity against three deadly mosquito vectors: , , and . We demonstrate that the deterrent active fraction isolated from replicate bacterial cultures is highly enriched in two compounds consistent with the previously described fabclavines, strongly suggesting that these are the molecular species responsible for feeding-deterrence. The mosquito feeding-deterrent activity in the putative fabclavine-rich fraction is comparable to or better than that of ,-diethyl-3-methylbenzamide (also known as DEET) or picaridin in side-by-side assays. These findings lay the groundwork for research into biologically derived, peptide-based, low-molecular weight compounds isolated from bacteria for exploitation as mosquito repellents and feeding-deterrents.
已鉴定出属于 属的昆虫病原细菌产生的细胞外次生代谢物具有抗生素和杀虫生物活性；然而，它们在蚊虫拒食等新应用方面尚未得到报道。在这里，我们表明，从 体外培养物中分离出的化合物混合物对三种致命的蚊子传播媒介： 、 、 和 表现出强烈的拒食活性。我们证明，从重复细菌培养物中分离出的驱避活性部分高度富含两种与先前描述的 fabclavines 一致的化合物，强烈表明这些是负责拒食的分子种类。在假定的 fabclavine 丰富部分中的蚊虫拒食活性与 、-二乙基-3-甲基苯甲酰胺（也称为 DEET）或派卡瑞丁在并排试验中相当或更好。这些发现为研究从细菌中分离出的基于生物的、肽基、低分子量化合物奠定了基础，这些化合物可作为蚊虫驱避剂和拒食剂加以利用。
