Ishizaka S, Kuriyama S, Tsujii T
Third Department of Internal Medicine, Nara Medical University, Japan.
J Immunol Methods. 1989 Nov 13;124(1):17-24. doi: 10.1016/0022-1759(89)90180-4.
Almost 90% of the sulfate groups of iota-carrageenan (CGN) was removed with acid-methanol in an attempt to obtain a product which would selectively eliminate macrophages in mice. Desulfated CGN(DS-CGN) failed to induce in vivo polyclonal antibody production in DBA/2 mice. However, the number of phagocytes in the peritoneal cavity, spleen, thymus and lymph node of DBA/2 mice was reduced stringently by DS-CGN treatment. The number of Mac-1 positive cells(macrophages) in DS-CGN-treated mice gradually decreased for at least 7 days after the last injection of DS-CGN. In contrast, the relative proportion of T and B lymphocytes in the lymphoid organs was unaffected by DS-CGN treatment. DS-CGN suppressed antibody responses to SRBC, a T cell and macrophage-dependent antigen, but no such suppressive effect was observed in the polyclonal antibody responses to LPS, a T cell and macrophage-independent B cell activator. Furthermore, the impaired SRBC antibody responses in DS-CGN-treated mice were restored following transfer of adherent cells but not T cells. These experimental results indicate that DS-CGN selectively eliminates macrophages without influencing lymphocyte function in vivo.
为了获得一种能选择性清除小鼠体内巨噬细胞的产物，用酸甲醇去除了近90%的iota-卡拉胶（CGN）中的硫酸基团。去硫酸化CGN（DS-CGN）未能在DBA/2小鼠体内诱导多克隆抗体产生。然而，DS-CGN处理能严格减少DBA/2小鼠腹腔、脾脏、胸腺和淋巴结中的吞噬细胞数量。在最后一次注射DS-CGN后至少7天内，经DS-CGN处理的小鼠中Mac-1阳性细胞（巨噬细胞）数量逐渐减少。相比之下，DS-CGN处理对淋巴器官中T和B淋巴细胞的相对比例没有影响。DS-CGN抑制了对SRBC（一种T细胞和巨噬细胞依赖性抗原）的抗体反应，但在对LPS（一种T细胞和巨噬细胞非依赖性B细胞激活剂）的多克隆抗体反应中未观察到这种抑制作用。此外，在转移黏附细胞而非T细胞后，DS-CGN处理小鼠中受损的SRBC抗体反应得以恢复。这些实验结果表明，DS-CGN在体内能选择性清除巨噬细胞而不影响淋巴细胞功能。
