Sun Yanan, Hu Xinlei, Li Wenxiang
Key Laboratory of Modern Agricultural Quality and Safety Engineering of Qingdao, Qingdao Agricultural University, Qingdao 266109, China.
Key Laboratory of Modern Agricultural Quality and Safety Engineering of Qingdao, Qingdao Agricultural University, Qingdao 266109, China.
Int J Biol Macromol. 2017 Apr;97:323-330. doi: 10.1016/j.ijbiomac.2017.01.043. Epub 2017 Jan 16.
The objective of this study was to investigate the antioxidant, antitumor and immunological activities of the polypeptide from Pleurotus eryngii mycelium (PEMP). The ability of the polypeptide to stimulate Ana-1 macrophages to englobe neutral red, secrete NO, HO, TNF-α, and IL-6, increase TLR2 and TLR4 expression levels and Ana-1 cell survival rate were investigated to study its immunocompetence. Its antitumor activities were studied by examining the survival of cervical, breast, and stomach cancer cells. The antioxidant activities of the polypeptide were studied by examining its reducing power and its ability to scavenge DPPH, O, and OH radicals. The results showed that the PEMP was a good antioxidant with antitumor and immunostimulatory activities that was concentration dependent. At concentrations from 0.05 to 2mg/mL, it inhibited the proliferation of cancer cells, but promoted the proliferation of macrophages, TNF-α and IL-6 secretion, TLR2 and TLR4 expression and increased macrophage phagocytic ability through NO and HO release. From 0.2 to 1mg/mL, the polypeptide had strong reducing power to clear free radical of DPPH, O, OH, with a concentration-response relationship. Based on these results, PEMP has potential applications in functional foods as a natural anti-aging and anti-virus agent with antioxidant and immunostimulatory activities.
本研究的目的是探讨杏鲍菇菌丝体多肽（PEMP）的抗氧化、抗肿瘤和免疫活性。通过研究该多肽刺激Ana-1巨噬细胞吞噬中性红、分泌NO、HO、TNF-α和IL-6、提高TLR2和TLR4表达水平以及Ana-1细胞存活率的能力，来研究其免疫活性。通过检测宫颈癌、乳腺癌和胃癌细胞的存活率来研究其抗肿瘤活性。通过检测该多肽的还原能力及其清除DPPH、O和OH自由基的能力来研究其抗氧化活性。结果表明，PEMP是一种具有抗肿瘤和免疫刺激活性的良好抗氧化剂，且具有浓度依赖性。在0.05至2mg/mL的浓度范围内，它抑制癌细胞的增殖，但促进巨噬细胞的增殖、TNF-α和IL-6的分泌、TLR2和TLR4的表达，并通过释放NO和HO提高巨噬细胞的吞噬能力。在0.2至1mg/mL的浓度范围内，该多肽对清除DPPH、O、OH自由基具有较强的还原能力，呈现浓度-效应关系。基于这些结果，PEMP作为一种具有抗氧化和免疫刺激活性的天然抗衰老和抗病毒剂，在功能性食品中具有潜在的应用价值。
