Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
Carbohydr Polym. 2019 Feb 15;206:706-715. doi: 10.1016/j.carbpol.2018.11.063. Epub 2018 Nov 20.
Mushroom-derived polysaccharides exhibit various biological activities owing to their diverse structural features. Here, we purified a 3-O-methylated heterogalactan (WPEP-N-b, Mw 21.4 kDa) from the fruiting bodies of Pleurotus eryngii. WPEP-N-b is composed primarily of galactose (43.8%), mannose (39.3%), methyl-galactose (11.7%) and glucose (9.2%) residues, with the main chain being composed of α-1,6-linked D-Galp and 3-O-Me-D-Galp, branched at O-2 with single t-β-D-Manp as major the side chain. β-1,6-D-Glcp residues are present as minor components either in side-chains or backbone. WPEP-N-b increases macrophage phagocytosis and secretion of NO, TNF-α, IL-6 and IL-1β. Mechanistic studies demonstrate that WPEP-N-b promotes the degradation of IκB-α, and enhances phosphorylation of MAPKs and the NF-κB p65 subunit. Our results also indicate that this polysaccharide activates RAW264.7 cells via MAPK and NF-κB signaling pathways and the Toll-like receptor 2(TLR2). These results increase our understanding as to how mushroom-derived polysaccharides modulate the immunologic process.
蘑菇来源的多糖由于其多样的结构特征而表现出各种生物活性。在这里，我们从杏鲍菇的子实体中纯化出一种 3-O-甲基化杂半乳糖（WPEP-N-b，Mw 21.4 kDa）。WPEP-N-b 主要由半乳糖（43.8%）、甘露糖（39.3%）、甲基半乳糖（11.7%）和葡萄糖（9.2%）残基组成，其主链由 α-1,6-连接的 D-Galp 和 3-O-Me-D-Galp 组成，在 O-2 处分支，主要侧链为单 t-β-D-Manp。β-1,6-D-Glcp 残基作为侧链或主链的次要成分存在。WPEP-N-b 增加了巨噬细胞的吞噬作用和 NO、TNF-α、IL-6 和 IL-1β 的分泌。机制研究表明，WPEP-N-b 促进 IκB-α 的降解，并增强 MAPKs 和 NF-κB p65 亚基的磷酸化。我们的结果还表明，这种多糖通过 MAPK 和 NF-κB 信号通路以及 Toll 样受体 2（TLR2）激活 RAW264.7 细胞。这些结果增加了我们对蘑菇来源的多糖如何调节免疫过程的理解。
