Department of Ophthalmology, The Fourth People's Hospital of Shenyang, Shenyang Eye Research Institute, Key Laboratory of Ophthalmology of Shenyang, China.
Department of Microbiology, The Chinese University of Hong Kong, Hong Kong.
Ocul Surf. 2017 Apr;15(2):242-247. doi: 10.1016/j.jtos.2016.12.003. Epub 2017 Jan 14.
To compare the bacteriological profile of meibomian gland secretion and conjunctival cul-de-sac in subjects with and without meibomian gland dysfunction (MGD).
This hospital-based study enrolled 201 eyes from 201 MGD patients and 84 eyes from 84 age- and gender-matched controls. Samples from the conjunctivae and meibomian gland secretion were obtained. Both aerobic and anaerobic cultures were performed. Differences in the culture-positivity and bacterial strains between two groups were compared.
36.9% and 10.7% of the control meibomian gland secretion recovered aerobes and anaerobes, respectively. A higher rate of aerobic (44.0%) and similar rate of anaerobic (10.7%) bacteria were isolated from the conjunctival cul-de-sac in these controls. Patients with MGD showed significantly higher positive culture rates from both the meibomian gland secretion (75.6% for aerobes and 34.3% for anaerobes) and conjunctival cul-de-sac (64.7% for aerobes and 30.8% for anaerobes). All p values were ≤ 0.001. In both groups and at either location, the predominant species isolated were Staphylococcus epidermidis (aerobes) and Propionibacterium acnes (anaerobes). Of note, the MGD patients harbor a much more complex bacterial profile than the controls.
Both aerobic and anaerobic bacteria can be isolated from the ocular surface of healthy subjects. Patients with MGD demonstrate significantly higher culture positivity and more complex bacterial profiles than the controls. Bacterial related cytotoxicity and/or inflammation may contribute to the pathological process of MGD.
比较患有和不患有睑板腺功能障碍（MGD）的受试者的睑板腺分泌物和结膜囊的细菌谱。
本项基于医院的研究纳入了 201 名 MGD 患者的 201 只眼和 84 名年龄和性别匹配的对照者的 84 只眼。采集结膜和睑板腺分泌物样本。进行需氧和厌氧培养。比较两组之间培养阳性和细菌菌株的差异。
对照组的睑板腺分泌物中分别有 36.9%和 10.7%回收了需氧菌和厌氧菌。对照组的结膜囊中有更高比例的需氧菌（44.0%）和相似比例的厌氧菌（10.7%）被分离出来。MGD 患者的睑板腺分泌物（需氧菌为 75.6%，厌氧菌为 34.3%）和结膜囊（需氧菌为 64.7%，厌氧菌为 30.8%）的阳性培养率均显著更高。所有 p 值均≤0.001。在两组和任意部位，分离出的主要菌种均为表皮葡萄球菌（需氧菌）和痤疮丙酸杆菌（厌氧菌）。值得注意的是，MGD 患者的细菌谱比对照组更为复杂。
健康受试者的眼表均可分离出需氧菌和厌氧菌。患有 MGD 的患者比对照组的培养阳性率更高，且细菌谱更为复杂。细菌相关的细胞毒性和/或炎症可能导致 MGD 的病理过程。
