Bai Wen L, Wang Jiao J, Yin Rong H, Dang Yun L, Wang Ze Y, Zhu Yu B, Cong Yu Y, Deng Liang, Guo Dan, Wang Shi Q, Yang Shu H, Xue Hui L
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
Institute of Animal Husbandry Science of Liaoning Province, Liaoyang, 130062, People's Republic of China.
Genetica. 2017 Feb;145(1):115-126. doi: 10.1007/s10709-017-9950-5. Epub 2017 Jan 16.
Homeobox protein Hox-C8 (HOXC8) is a member of Hox family. It is expressed in the dermal papilla of the skin and is thought to be associated with the hair inductive capacity of dermal papilla cells. In the present study, we isolated and characterized a full-length open reading frame of HOXC8 cDNA from the skin tissue of Liaoning cashmere goat, as well as, established a phylogenetic relationship of goat HOXC8 with that of other species. Also, we investigated the effect of methylation status of HOXC8 exon 1 at anagen secondary hair follicle on the cashmere fiber traits in Liaoning cashmere goat. The sequence analysis indicated that the obtained cDNA was 1134-bp in length containing a complete ORF of 729-bp. It encoded a peptide of 242 amino acid residues in length. The structural analysis indicated that goat HOXC8 contained a typical homeobox domain. The phylogenetic analysis revealed that Capra hircus HOXC8 had a closer genetic relationship with that of Ovis aries, followed by Bos Taurus and Bubalus bubalis. The methylation analysis suggested that the methylation degree of HOXC8 exon 1 in anagen secondary hair follicle might be involved in regulating the growth of cashmere fiber in Liaoning cashmere goat. Our results provide new evidence for understanding the molecular structural and evolutionary characteristics of HOXC8 in Liaoning cashmere goat, as well as, for further insight into the role of methylation degree of HOXC8 exon 1 regulates the growth of cashmere fiber in goat.
同源框蛋白Hox-C8（HOXC8）是Hox家族的成员。它在皮肤的真皮乳头中表达，被认为与真皮乳头细胞的毛发诱导能力有关。在本研究中，我们从辽宁绒山羊的皮肤组织中分离并鉴定了HOXC8 cDNA的全长开放阅读框，并建立了山羊HOXC8与其他物种的系统发育关系。此外，我们研究了生长期次级毛囊中HOXC8外显子1的甲基化状态对辽宁绒山羊羊绒纤维性状的影响。序列分析表明，获得的cDNA长度为1134bp，包含一个729bp的完整开放阅读框。它编码一个长度为242个氨基酸残基的肽。结构分析表明，山羊HOXC8包含一个典型的同源框结构域。系统发育分析显示，山羊HOXC8与绵羊的遗传关系更近，其次是牛和水牛。甲基化分析表明，生长期次级毛囊中HOXC8外显子1的甲基化程度可能参与调控辽宁绒山羊羊绒纤维的生长。我们的结果为理解辽宁绒山羊HOXC8的分子结构和进化特征提供了新证据，也为进一步深入了解HOXC8外显子1的甲基化程度对山羊羊绒纤维生长的调控作用提供了依据。
