College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, P. R. China.
Sichuan Animal Science Academy, Chengdu, P. R. China.
Anim Biotechnol. 2020 Apr;31(2):122-134. doi: 10.1080/10495398.2018.1553788. Epub 2019 Jan 11.
Long noncoding RNAs (lncRNAs), a class of non-protein conding RNAs > 200 nt in length, were thought to play critical roles in regulating the expression of protein-coding genes. Here, we identified and characterized a novel lncRNA-000133 from the secondary hair follicle (SHF) of cashmere goat with its ceRNA network analysis, as well as, its potential effects on inductive property of dermal papilla cells were evaluated through overexpression analysis. Expression analysis indicated that lncRNA-000133 had a significantly higher expression at anagen than that at telogen in SHF of Cashmere goat, suggesting that lncRNA-000133 might be involved in the reconstruction of SHF with the formation and growth of cashmere fiber. Taken together with methylation analysis, we showed that 5' regulatory region methylation of the lncRNA-000133 gene might be involved in its expression suppression in SHF of Cashmere goat. The ceRNA regulatory network showed that a rich and complex regulatory relationship between lncRNA-000133 and related miRNAs with their target genes. The overexpression of lncRNA-000133 led to a significant increasing in the relative expression of ET-1, SCF, ALP and LEF1 in dermal papilla cells suggesting that lncRNA-000133 appears to contribute the inductive property of dermal papilla cells.
长链非编码 RNA（lncRNA）是一类长度大于 200nt 的非蛋白编码 RNA，被认为在调节蛋白编码基因的表达中发挥着关键作用。在这里，我们从绒山羊次生毛囊（SHF）中鉴定并表征了一个新的 lncRNA-000133，并对其 ceRNA 网络进行了分析，同时通过过表达分析评估了其对真皮乳头细胞诱导特性的潜在影响。表达分析表明，lncRNA-000133 在 SHF 的生长期（anagen）的表达显著高于休止期（telogen），表明 lncRNA-000133 可能参与了 SHF 的重建，与羊绒纤维的形成和生长有关。结合甲基化分析，我们表明 lncRNA-000133 基因的 5'调控区甲基化可能参与了其在绒山羊 SHF 中的表达抑制。ceRNA 调控网络显示，lncRNA-000133 与其相关 miRNAs 及其靶基因之间存在丰富而复杂的调控关系。lncRNA-000133 的过表达导致真皮乳头细胞中 ET-1、SCF、ALP 和 LEF1 的相对表达显著增加，表明 lncRNA-000133 似乎有助于真皮乳头细胞的诱导特性。
