Department of Analytical Chemistry; Reference Materials, BAM Federal Institute for Materials Research and Testing , Richard-Willstätter-Str. 11, D-12489 Berlin, Germany.
Department of Organic Chemistry, University Autónoma of Madrid , Cantoblanco, 28049 Madrid, Spain.
Langmuir. 2017 Feb 7;33(5):1197-1201. doi: 10.1021/acs.langmuir.6b03855. Epub 2017 Jan 27.
We report for the first time the formation of site-specific interstrand cross-linked (ICL) surface-immobilized furan-modified DNA duplexes via singlet oxygen. O, necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as a photosensitizer. Via surface plasmon resonance spectroscopy, we show that surface ICL was achieved, and a robust link formed that enhances the stability of the 12-mer duplex even after surface regeneration. The described method represents a novel platform technology based on surfaces with addressable and stable DNA duplexes requiring only short oligonucleotides.
我们首次报道了通过单线态氧形成的、具有特定位置的链间交联（ICL）的表面固定呋喃修饰的 DNA 双链体。单线态氧 O 2 是形成呋喃介导的 ICL 所必需的，通过使用亚甲基蓝或锌酞菁衍生物（TT1）作为光敏剂原位产生。通过表面等离子体共振光谱法，我们表明实现了表面 ICL，并且形成了稳定的键，即使在表面再生后，也增强了 12 mer 双链体的稳定性。所描述的方法代表了一种基于具有可寻址和稳定 DNA 双链体的表面的新型平台技术，该技术仅需要短寡核苷酸。
