Laboratory for Organic and Biomimetic Chemistry, University of Ghent, Krijgslaan 281 S4, B-9000 Ghent, Belgium.
J Am Chem Soc. 2012 Jul 4;134(26):10737-40. doi: 10.1021/ja301901p. Epub 2012 Jun 25.
A new biocompatible strategy for photoinduced DNA interstrand cross-linking is presented. Methylene blue induced (1)O(2) formation triggers furan oxidation; the resulting aldehyde then rapidly reacts with complementary A or C with formation of stable adducts. Easily accessible furan modified nucleosides, a commercially available photosensitizer, and visible light irradiation constitute the necessary tools to achieve selective duplex interstrand cross-linking.
提出了一种新的光诱导 DNA 链间交联的生物相容性策略。亚甲基蓝诱导（1）O2 的形成引发呋喃氧化；生成的醛随后迅速与互补的 A 或 C 反应，形成稳定的加合物。易于获得的呋喃修饰核苷、一种市售的光敏剂和可见光照射构成了实现选择性双链间交联的必要工具。
