a Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía, Mexico City 14269, Mexico.
b Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa (UNIPAMPA), Uruguaiana, Rio Grande do Sul 97500-970, Brazil.
Appl Physiol Nutr Metab. 2017 Nov;42(11):1172-1178. doi: 10.1139/apnm-2017-0267. Epub 2017 Jul 14.
Immobilization induces oxidative damage to the brain. Ilex paraguariensis extracts (Mate) and their major natural compound, chlorogenic acid (CGA), exert protective effects against reactive oxygen species formation. Here, the effects of Mate and CGA on oxidative damage induced by chronic immobilization stress (CIS) in the cortex, hippocampus, and striatum were investigated. For CIS, animals were immobilized for 6 h every day for 21 consecutive days. Rats received Mate or CGA by intragastric gavage 30 min before every restraint session. Endpoints of oxidative stress (levels of lipid peroxidation, protein carbonylation, and reduced (GSH) and oxidized (GSSG) forms of glutathione) were evaluated following CIS. While CIS increased oxidized lipid and carbonyl levels in all brain regions, CGA (and Mate to a lesser extent) attenuated lipid and protein oxidation as compared with control groups. GSH/GSSG balance showed a tendency to increase in all regions in response to stress and antioxidants. Taken together, our results support a protective role of dietary antioxidants against the neuronal consequences of stress.
固定可诱导大脑氧化损伤。巴拉圭冬青提取物（马黛茶）及其主要天然化合物绿原酸（CGA）可抵抗活性氧的形成，发挥保护作用。在此，研究了马黛茶和 CGA 对慢性固定应激（CIS）诱导的大脑皮质、海马和纹状体氧化损伤的影响。对于 CIS，动物每天固定 6 小时，连续 21 天。在每次束缚前 30 分钟，大鼠通过灌胃接受马黛茶或 CGA。CIS 后评估氧化应激终点（脂质过氧化、蛋白质羰基化以及还原型（GSH）和氧化型（GSSG）谷胱甘肽水平）。虽然 CIS 增加了所有脑区的氧化脂质和羰基水平，但与对照组相比，CGA（马黛茶的作用较小）可减轻脂质和蛋白质氧化。GSH/GSSG 平衡在所有区域中均表现出对压力和抗氧化剂的增加趋势。综上所述，我们的结果支持膳食抗氧化剂对应激引起的神经元后果的保护作用。
