Kobayashi R, Matsuzawa S
Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
J Forensic Sci. 1989 Sep;34(5):1078-81.
An asymmetric ribonucleic acid (RNA) probe, which represents a Y-chromosome specific nucleotide sequence, was applied to the sex origin of bloodstains by using a nonisotopic Southern hybridization method. The RNA probe was in vitro transcribed from the recombinant of the Y-chromosome specific deoxyribonucleic acid (DNA) sequence and transcription-vector Bluescript SK M13+. The use of this RNA probe can lower the detection limit of the nonisotopic DNA analyzing method to 1/8 of that reached by our previously reported method in which a DNA probe was used.
一种代表Y染色体特异性核苷酸序列的不对称核糖核酸（RNA）探针，通过非同位素Southern杂交方法应用于血迹的性别鉴定。该RNA探针是从Y染色体特异性脱氧核糖核酸（DNA）序列与转录载体pBluescript SK M13 +的重组体进行体外转录获得的。使用这种RNA探针可将非同位素DNA分析方法的检测限降低至我们先前报道的使用DNA探针方法的1/8。
