Ho Derek, Fereidouni Farzad, Levenson Richard M, Jagdeo Jared
J Drugs Dermatol. 2016 Nov 1;15(11):1344-1346.
Skin care products make up the largest part (36%) of the cosmetic market globally, of which the United States plays the largest role. In 2015, approximately 115 billion USD was spent globally on skin care products. Skin care products, in contradistinction to pharmaceuticals, are not strictly regulated by the FDA. A key factor for evaluation of a skin care product or topical drug is skin barrier function and effect on super cial skin. Thus, it is critical to have quantitative and qualitative methods to study the effects of skin care products on skin barrier and the super cial skin. Currently, no imaging method exists that can evaluate and track super cial skin changes visually in real-time.
To report using a novel imaging modality, Microscopy using Ultraviolet Surface Excitation (MUSE), to provide real-time, high- resolution, in vivo characterization of super cial skin and moisturizing properties of topical moisturizer, and to highlight key bene ts of using MUSE to visualize the super cial skin and serve as an excellent complementary tool to current quantitative methods.
The methodology of MUSE is based upon two main principles inherent to ultraviolet (UV) light and uorescent staining agents. In this study, the author's (JJ) index ngertip was imaged using the MUSE instrument without and with moisturizer.
Dermatoglyphics of the fingertip consists of ridges (cristae super ciales) and grooves (sulci super ciales) proved to be straightforward to visualize at high resolution. Desquamation of superficial corneocytes and opening of an acrosyringium (the most superficial portion of eccrine ducts) were visualized in high-resolution. Post-application of a moisturizer, a uniform layer of moisturizer could be seen superficial to the corneocytes along the ridges and CONCLUSIONS: Real-time, high-resolution, in vivo characterization of super cial skin and moisturizing properties of moisturizer using MUSE is feasible. Its utility can be enhanced with downstream quantification using imaging software. J Drugs Dermatol. 2016;15(11):1344-1346..
护肤品在全球化妆品市场中占比最大（36%），其中美国占据最大份额。2015年，全球在护肤品上的花费约为1150亿美元。与药品不同，护肤品不受美国食品药品监督管理局（FDA）的严格监管。评估护肤品或局部用药的一个关键因素是皮肤屏障功能以及对表皮的影响。因此，拥有定量和定性方法来研究护肤品对皮肤屏障和表皮的影响至关重要。目前，尚无成像方法能够实时直观地评估和追踪表皮变化。
报告使用一种新型成像方式——紫外线表面激发显微镜（MUSE），对表皮进行实时、高分辨率的体内特征分析以及局部保湿剂的保湿特性分析，并强调使用MUSE可视化表皮的关键优势，以及其作为当前定量方法的优秀补充工具的作用。
MUSE的方法基于紫外线（UV）光和荧光染色剂固有的两个主要原理。在本研究中，使用MUSE仪器对作者（JJ）的食指指尖在涂抹保湿剂前后进行成像。
指尖的皮纹由嵴（表皮嵴）和沟（表皮沟）组成，高分辨率下很容易可视化。高分辨率下可观察到浅表角质形成细胞的脱屑和小汗腺导管最浅表部分（顶泌汗腺）的开口。涂抹保湿剂后，沿嵴可在角质形成细胞表面看到一层均匀的保湿剂。
使用MUSE对表皮进行实时、高分辨率的体内特征分析以及保湿剂的保湿特性分析是可行的。通过使用成像软件进行下游定量分析，其效用可以得到增强。《药物皮肤病学杂志》。2016年；15（11）：1344 - 1346。
