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来自印度[具体地点未提及]的[线虫纲:旋尾科]的分子特征分析

Molecular Characterization of (Nematoda: Heligmosomatidae) from in India.

作者信息

Chaudhary Anshu, Goswami Urvashi, Singh Hridaya Shanker

机构信息

Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), 250004, India.

出版信息

Korean J Parasitol. 2016 Dec;54(6):743-750. doi: 10.3347/kjp.2016.54.6.743. Epub 2016 Dec 31.

DOI:10.3347/kjp.2016.54.6.743
PMID:28095659
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5266368/
Abstract

(Rodentia: Muridae) has generally been infected with a rodent hookworm . In this report, we present morphological and molecular identification of by light and scanning electron microscopy and PCR amplification of mitochondrial cytochrome c oxidase subunit 1 () gene and the protein sequences encoded by gene, respectively. Despite the use of in many biochemistry studies from India, their taxonomic identification was not fully understood, especially at the species level, and no molecular data is available in GenBank from India. Sequence analysis of gene in this study revealed that the present specimen showed close identity with the same species available in GenBank, confirming that the species is . This study represents the first record of molecular identification of from India and the protein structure to better understand the comparative phylogenetic characteristics.

摘要

(啮齿目:鼠科)通常感染了一种鼠类钩虫。在本报告中,我们分别通过光学显微镜和扫描电子显微镜以及线粒体细胞色素c氧化酶亚基1()基因的PCR扩增和该基因编码的蛋白质序列,对[未提及具体物种名称]进行了形态学和分子鉴定。尽管在印度的许多生物化学研究中使用了[未提及具体物种名称],但其分类鉴定尚未完全明确,尤其是在物种水平,并且印度的GenBank中没有相关分子数据。本研究中对[未提及具体物种名称]基因的序列分析表明,当前标本与GenBank中可获得的同一物种具有密切的同一性,证实该物种为[未提及具体物种名称]。本研究代表了来自印度的[未提及具体物种名称]分子鉴定及蛋白质结构的首次记录,以便更好地了解比较系统发育特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/a3c6503bb961/kjp-54-6-743f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/825d39bdf178/kjp-54-6-743f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/e27a2a5df764/kjp-54-6-743f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/0966c45b58f5/kjp-54-6-743f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/1edc397991c4/kjp-54-6-743f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/b2210ff59f01/kjp-54-6-743f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/7e4203614b35/kjp-54-6-743f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/6e9309439bf0/kjp-54-6-743f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/a3c6503bb961/kjp-54-6-743f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/825d39bdf178/kjp-54-6-743f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/e27a2a5df764/kjp-54-6-743f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/0966c45b58f5/kjp-54-6-743f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/1edc397991c4/kjp-54-6-743f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/b2210ff59f01/kjp-54-6-743f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/7e4203614b35/kjp-54-6-743f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/6e9309439bf0/kjp-54-6-743f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d976/5266368/a3c6503bb961/kjp-54-6-743f8.jpg

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