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一只华南虎线粒体基因组的序列分析

Sequence Analysis of Mitochondrial Genome of from a South China Tiger.

作者信息

Li Kangxin, Yang Fang, Abdullahi A Y, Song Meiran, Shi Xianli, Wang Minwei, Fu Yeqi, Pan Weida, Shan Fang, Chen Wu, Li Guoqing

机构信息

College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.

Guangzhou Zoo, Guangdong Province, 510075, People's Republic of China.

出版信息

Korean J Parasitol. 2016 Dec;54(6):803-807. doi: 10.3347/kjp.2016.54.6.803. Epub 2016 Dec 31.

Abstract

is a common parasitic nematode of wild mammals and has significant impacts on the protection of rare wild animals. To analyze population genetic characteristics of from South China tiger, its mitochondrial (mt) genome was sequenced. Its complete circular mt genome was 14,277 bp in length, including 12 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions. The nucleotide composition was biased toward A and T. The most common start codon and stop codon were TTG and TAG, and 4 genes ended with an incomplete stop codon. There were 13 intergenic regions ranging 1 to 10 bp in size. Phylogenetically, from a South China tiger was close to canine . This study reports for the first time a complete mt genome sequence of from the South China tiger, and provides a scientific basis for studying the genetic diversity of nematodes between different hosts.

摘要

是野生哺乳动物常见的寄生线虫,对珍稀野生动物保护具有重大影响。为分析华南虎体内该线虫的群体遗传特征,对其线粒体(mt)基因组进行了测序。其完整的环状mt基因组长度为14277 bp,包括12个蛋白质编码基因、22个tRNA基因、2个rRNA基因和2个非编码区。核苷酸组成偏向于A和T。最常见的起始密码子和终止密码子分别为TTG和TAG,4个基因以不完全终止密码子结尾。有13个基因间隔区,大小在1至10 bp之间。在系统发育上,华南虎体内的该线虫与犬类的该线虫亲缘关系较近。本研究首次报道了华南虎体内该线虫的完整mt基因组序列,为研究不同宿主中线虫的遗传多样性提供了科学依据。

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