Mahmoudvand Hossein, Sheibani Vahid, Keshavarz Hossein, Shojaee Saeedeh, Esmaeelpour Khadijeh, Ziaali Naser
Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Parasitol. 2016 Apr-Jun;11(2):177-185.
Here, we established the mouse models of chronic toxoplasmosis by Tehran strain to provide a good understanding about defining the possible association between exposure and learning and memory impairments. Moreover, as secondary objective of the present study, we hypothesized whether administration of an acetylcholinesterase (AChE) inhibitor could reduce learning and memory impairments induced by infection.
Twenty-four male BALB/c mice were used to establishment of latent toxoplasmosis. The animal model of infection was established by the intraperitoneal inoculation of 20-25 tissue cysts from Tehran strain of Donepezil (2 mg/kg) an AChE inhibitor to treat Alzheimer disease was injected intraperitoneally once a day for two weeks starting from post-infection day 90. Morris water maze (MWM) task was used to assay spatial learning and short term spatial memory in all groups. One-way ANOVA with Tukey's post-hoc test was used to assess differences between experimental groups. <0.05 was considered statistically significant.
infection impaired spatial leaning and short term spatial memory of the infected BALB/c mice, whereas donepezil, an AChE inhibitor, improved impairments induced by infection.
infection through increasing AChE reduces the level of Acetylcholine (Ach) and consequently affects learning and memory activity in infected hosts, whereas, donepezil as an AChE inhibitor improves these impairments by restoring ACh levels at synapses of neurons in brain.
在此，我们通过德黑兰株建立了慢性弓形虫病小鼠模型，以更好地理解暴露与学习和记忆障碍之间可能存在的关联。此外，作为本研究的次要目标，我们假设给予乙酰胆碱酯酶（AChE）抑制剂是否可以减轻感染引起的学习和记忆障碍。
使用24只雄性BALB/c小鼠建立潜伏性弓形虫病模型。通过腹腔接种来自德黑兰株的20 - 25个组织包囊来建立感染动物模型。从感染后第90天开始，每天腹腔注射一次多奈哌齐（一种用于治疗阿尔茨海默病的AChE抑制剂，剂量为2 mg/kg），持续两周。使用莫里斯水迷宫（MWM）任务来测定所有组的空间学习和短期空间记忆。采用单因素方差分析和Tukey事后检验来评估实验组之间的差异。P < 0.05被认为具有统计学意义。
感染损害了受感染BALB/c小鼠的空间学习和短期空间记忆，而AChE抑制剂多奈哌齐改善了感染引起的损伤。
感染通过增加AChE降低乙酰胆碱（Ach）水平，从而影响受感染宿主的学习和记忆活动，而多奈哌齐作为AChE抑制剂通过恢复大脑神经元突触处的Ach水平来改善这些损伤。
