Shakibaie Mojtaba, Ezzatkhah Fatemeh, Gabal Esraa, Badparva Ebrahim, Jahanbakhsh Sareh, Mahmoudvand Hossein
Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Laboratory Sciences, Sirjan School of Medical Sciences, Sirjan, Iran.
Ann Med Surg (Lond). 2020 Apr 29;54:85-88. doi: 10.1016/j.amsu.2020.04.010. eCollection 2020 Jun.
In this investigation, the in vivo efficacy and safety of biogenic selenium nanoparticles (SeNPs) are assessed against acute toxoplasmosis caused by (Sarcocystidae) in the mice.
Male NMRI mice were orally treated with normal saline (control group) and SeNPs at the doses of 5 and 10 mg/kg once a day for 14 days On the 15th day, the mice were infected with 10 tachyzoites of RH strain by the intraperitoneal route. The mortality rate and parasite load were determined in the infected mice. The mRNA levels of IFN-γ, IL10, IL12, and inducible nitric oxide synthase were also examined in the infected mice by quantitative real-time PCR.
The rate of mortality in the infected mice receiving SeNPs at the doses of 5 and 10 mg/kg compared with the mice in the control group was 100% on the 9 and 10 days after the administration. The mean number of tachyzoites in the infected mice receiving SeNPs was significantly lower than that in the control group. No significant difference ( > 0.05) was found in the biochemical parameters between the mice treated with SeNPs and the mice in the control group. The results revealed that mRNA levels significantly improved in the infected mice treated with SeNPs compared with those in the control group.
Findings of the present investigation showed the considerable efficacy of SeNPs with no important toxicity for curing acute toxoplasmosis in the mice model. However, further studies are needed to clarify the accurate anti-Toxoplasma mechanisms of SeNPs.
在本研究中，评估了生物源硒纳米颗粒（SeNPs）对小鼠由（肉孢子虫科）引起的急性弓形虫病的体内疗效和安全性。
雄性NMRI小鼠口服生理盐水（对照组）和剂量为5和10mg/kg的SeNPs，每天一次，持续14天。在第15天，通过腹腔途径用10个RH株速殖子感染小鼠。测定感染小鼠的死亡率和寄生虫载量。还通过定量实时PCR检测感染小鼠中IFN-γ、IL10、IL12和诱导型一氧化氮合酶的mRNA水平。
与对照组小鼠相比，接受5和10mg/kg剂量SeNPs的感染小鼠在给药后第9天和第10天的死亡率为100%。接受SeNPs的感染小鼠中的速殖子平均数量显著低于对照组。接受SeNPs治疗的小鼠与对照组小鼠之间的生化参数无显著差异（P>0.05）。结果显示，与对照组相比，接受SeNPs治疗的感染小鼠的mRNA水平显著提高。
本研究结果表明，SeNPs在治疗小鼠急性弓形虫病方面具有显著疗效且无重要毒性。然而，需要进一步研究以阐明SeNPs准确的抗弓形虫机制。
