Gholami Shirzad, Jahandar Hefzallah, Abastabar Mahdi, Pagheh Abdolsatar, Mobedi Iraj, Sharbatkhori Mitra
Department of Parasitology & Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Iran J Parasitol. 2016 Apr-Jun;11(2):186-194.
The aim of the present study was genotyping of isolates from dogs and jackals in Mazandaran Province, northern Iran, and using partial sequence of the mitochondrial cytochrome c oxidase subunit 1 gene ().
isolates (n = 15) were collected from 42 stray dogs and 16 jackals found in south of the Caspian Sea in northern Iran. After morphological study, the isolates were genetically characterized using consensus sequences (366bp) of the gene. Phylogenetic analysis of nucleotide sequence data was performed using a Bayesian Inference approach.
Four different sequences were observed among the isolates. Two genotypes [G1 (66.7%) and G3 (33.3%)] were identified among the isolates. The G1 sequences indicated three sequence profiles. One profile (Maz1) had 100% homology with reference sequence (AN: KP339045). Two other profiles, designated Maz2 and Maz3, had 99% homology with the G1 genotype (ANs: KP339046 and KP339047). A G3 sequence designated Maz4 showed 100% homology with a G3 reference sequence (AN: KP339048).
The occurrence of the G1 genotype of as a frequent genotype in dogs is emphasized. This study established the first molecular characterization of in the province.
本研究的目的是对伊朗北部马赞德兰省狗和豺的分离株进行基因分型，并使用线粒体细胞色素c氧化酶亚基1基因（）的部分序列。
从伊朗北部里海南部发现的42只流浪狗和16只豺中收集了15株分离株。经过形态学研究后，使用该基因的共有序列（366bp）对分离株进行基因特征分析。使用贝叶斯推断方法对核苷酸序列数据进行系统发育分析。
在分离株中观察到四种不同的序列。在分离株中鉴定出两种基因型[G1（66.7%）和G3（33.3%）]。G1序列显示出三种序列图谱。一种图谱（Maz1）与参考序列（登录号：KP339045）具有100%的同源性。另外两种图谱，命名为Maz2和Maz3，与G1基因型（登录号：KP339046和KP339047）具有99%的同源性。一个命名为Maz4的G3序列与一个G3参考序列（登录号：KP339048）具有100%的同源性。
强调了作为狗中常见基因型的G1基因型的出现。本研究建立了该省的首次分子特征分析。
