Neysi Hamidreza, Mohammadzadeh Tahereh, Sadjjadi Seyed Mahmoud, Akhavanmoghaddam Jamal, Shamsaei Alireza
Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2020 Jan-Mar;15(1):130-137.
Cystic echinococcosis (CE) is a worldwide zoonotic helminthic disease caused by the larval stage of . The infection is particularly important in terms of economic and medico-veterinary aspects in endemic areas including Iran. Considering the possibility of organ-tropism in strains, the present study was aimed to identify the genotypes of in different organs involved in patients, undergone surgery in Baqiyatallah Hospital, Tehran, Iran from 2005-2015.
Overall, 29 formalin-fixed paraffin-embedded tissues (FFPT) from patients with histologically confirmed CE including liver (N: 14) lungs (N: 6) abdomen (N: 2), pancreas (N: 2) and each of spleen, gallbladder and, muscles (N: 1) plus unknown organs (N: 2) were used and genetically characterized using polymerase chain reaction, followed by partial sequencing of mitochondrial cytochrome c oxidase gene subunit 1(cox1) and analyzed.
Nineteen out of 29 isolates including liver (N: 6) lungs (N: 4) abdomen (N: 2), pancreas (N: 2) and each of spleen, gallbladder, and muscle (N: 1), unknown organs (N: 2) obtained from paraffin-embedded blocks of human CE created an acceptable sequence in two directions. All 19 isolates regardless of the organ involved were recognized as sensu stricto (G1).
The sequence alignments of the isolates displayed two profiles. All sequenced samples showed sensu stricto (G1) with no organ-related genotype.
囊型包虫病（CE）是一种由 的幼虫阶段引起的全球性人畜共患蠕虫病。在包括伊朗在内的流行地区，该感染在经济和医学 - 兽医方面尤为重要。考虑到 菌株存在器官嗜性的可能性，本研究旨在鉴定2005 - 2015年在伊朗德黑兰巴奇亚塔拉医院接受手术的患者不同受累器官中 的基因型。
总体而言，使用了29份来自组织学确诊为CE患者的福尔马林固定石蜡包埋组织（FFPT），包括肝脏（n = 14）、肺（n = 6）、腹部（n = 2）、胰腺（n = 2）以及脾脏、胆囊和肌肉各1份（n = 1），还有未知器官2份（n = 2），采用聚合酶链反应进行基因特征分析，随后对线粒体细胞色素c氧化酶基因亚基1（cox1）进行部分测序并分析。
从人类CE石蜡包埋块中获得的29个分离株中的19个，包括肝脏（n = 6）、肺（n = 4）、腹部（n = 2）、胰腺（n = 2）以及脾脏、胆囊和肌肉各1份（n = 1）、未知器官2份（n = 2），在两个方向上都产生了可接受的序列。所有19个分离株，无论涉及的器官如何，均被鉴定为狭义 （G1）。
分离株的序列比对显示出两种模式。所有测序样本均显示为狭义 （G1），无器官相关基因型。
