聚焦狭义复合群研究伊朗北部里海沿岸地区人类包虫病的系统发育地理学与遗传多样性
Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Sensu Stricto Complex.
作者信息
Siyadatpanah Abolghasem, Daryani Ahmad, Sarvi Shahabeddin, Spotin Adel, Sharif Mehdi, Esmaeelzadeh Dizaji Reza, Anvari Davood, Emami Zeydi Amir, Kohansal Mohammad Hasan, Agudelo Higuita Nelson Ivan, Hosseini Seyed Abdollah, Aryaeipour Mojgan, Gholami Shirzad
机构信息
Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran.
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
出版信息
Iran J Public Health. 2020 Sep;49(9):1758-1768. doi: 10.18502/ijph.v49i9.4097.
BACKGROUND
Human a cyclo-zoonotic infection caused by tapeworms of the sensu stricto complex. The detection of mitochondrial genome data of genus can reflect the taxonomic status, genetic diversity, and population structure genetics.
METHODS
Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 () gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity.
RESULTS
Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified.
CONCLUSION
G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.
背景
人类囊型包虫病是由狭义细粒棘球绦虫复合体的绦虫引起的一种环动物共患感染。细粒棘球绦虫属线粒体基因组数据的检测可以反映其分类地位、遗传多样性和群体结构遗传学。
方法
1995年3月至2018年5月期间，从伊朗马赞德兰省收集了52份经组织学确诊为囊型包虫病患者的福尔马林固定石蜡包埋（FFPE）组织样本。所有从（FFPE）组织样本中提取的DNA均通过针对细胞色素c氧化酶亚基1（CO1）基因的聚合酶链反应方法进行扩增。所有PCR扩增产物均进行测序以进行系统发育分析和遗传多样性分析。
结果
分子分析表明，分别有50株（96.1%）和2株（3.84%）分离株被鉴定为G1和G3基因型。DNA序列分析表明G1（单倍型多样性：0.830）和G3基因型（Hd：1.00）具有较高的基因多样性。基于多序列比对分析，明确鉴定出7种（13.46%；G1基因型）和2种（3.84%；G3基因型）新的单倍型。
结论
从该地区两例人类包虫病分离株中鉴定出G3基因型（水牛株）。本研究加强了我们对该寄生虫在伊朗北部临床囊型包虫病分离株中的分类地位、向人类的传播模式以及异质性方面的认识。
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