Fu Pin-Kuei, Pan Tai-Long, Yang Chi-Yu, Jeng Kee-Ching, Tang Nou-Ying, Hsieh Ching-Liang
Department of Critical Care Medicine, Taichung Veterans General Hospital, Taichung, 40705, Taiwan.
School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
Iran J Basic Med Sci. 2016 Dec;19(12):1368-1375. doi: 10.22038/ijbms.2016.7925.
L. (CT) or safflower is widely used in traditional Chinese medicine. This study investigated the effects of CT extract (CTE) on ischemia-reperfusion (I/R) brain injury and elucidated the underlying mechanism.
The I/R model was conducted by occlusion of both common carotid arteries and right middle cerebral artery for 90 min followed by 24 hr reperfusion in Sprague-Dawley rats. CTE (0.2-0.6 g/kg) was administered intraperitoneally before and during ischemia, and during reperfusion period. The cerebral infarction area, neurological deficit scores, free radicals (lucigenin chemiluminescence counts) and pro-inflammatory cytokines expression were measured.
Pretreatment and treatment with CTE significantly reduced the cerebral infarction area and neurological deficits. CTE (0.4 g/kg) also reduced blood levels of free radicals and expression of tumor necrosis factor-α and interleukin-1β in the cerebral infarction area.
The reduction in I/R cerebral infarction caused by CTE is possibly associated with its antioxidation and anti-inflammatory properties.
川芎（CT）或红花在传统中药中广泛应用。本研究调查了川芎提取物（CTE）对缺血再灌注（I/R）脑损伤的影响，并阐明其潜在机制。
在Sprague-Dawley大鼠中，通过夹闭双侧颈总动脉和右侧大脑中动脉90分钟，随后再灌注24小时建立I/R模型。在缺血前、缺血期间以及再灌注期间腹腔注射CTE（0.2 - 0.6克/千克）。测量脑梗死面积、神经功能缺损评分、自由基（光泽精化学发光计数）和促炎细胞因子表达。
CTE预处理和治疗显著减小了脑梗死面积并改善了神经功能缺损。CTE（0.4克/千克）还降低了脑梗死区域的自由基血水平以及肿瘤坏死因子-α和白细胞介素-1β的表达。
CTE引起的I/R脑梗死面积减小可能与其抗氧化和抗炎特性有关。
